UBE3A Recombinant Rabbit Monoclonal Antibody [JE32-48]
cat.: HA721921
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JE32-48 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 101 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human UBE3A aa 826-875 / 875. |
| Positive control: | K-562 cell lysate, HEK-293 cell lysate, Jurkat cell lysate. |
| Subcellular location: | Nucleus. Cytoplasm. |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: Q05086 Human |
| Alternative names: | ANCR Angelman syndrome AS CTCL tumor antigen se37 2 CTCL tumor antigen se37-2 E6 AP E6AP E6AP ubiquitin protein ligase E6AP ubiquitin-protein ligase EPVE6AP FLJ26981 HECT-type ubiquitin transferase E3A HPVE6A Human papilloma virus E6 associated protein Angelman syndrome Human papilloma virus E6 associated protein Human papillomavirus E6-associated protein NY REN 54 antigen Oncogenic protein associated protein E6 AP Oncogenic protein associated protein E6AP Oncogenic protein-associated protein E6-AP Renal carcinoma antigen NY REN 54 Renal carcinoma antigen NY-REN-54 UBE 3A Ube3a UBE3A protein UBE3A_HUMAN Ubiquitin protein ligase E3A Ubiquitin-protein ligase E3A |
Images
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Fig1:
Western blot analysis of UBE3A on different lysates with Rabbit anti-UBE3A antibody (HA721921) at 1/1,000 dilution. Lane 1: K-562 cell lysate Lane 2: HEK-293 cell lysate Lane 3: Jurkat cell lysate Lysates/proteins at 30 µg/Lane. Predicted band size: 101 kDa Observed band size: 101 kDa Exposure time: 1 minute 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721921) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.