GRB2 Recombinant Rabbit Monoclonal Antibody [JE49-73]
cat.: HA721923
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat, Monkey |
| Applications: | WB, IF-Cell, FC |
| Clonality: | Monoclonal |
| Clone number: | JE49-73 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 25 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human GRB2 aa 1-100 / 217. |
| Positive control: | HeLa cell lysate, 293T cell lysate, A431 cell lysate, MCF7 cell lysate, HCT 116 cell lysate, COS-1 cell lysate, RAW264.7 cell lysate, NIH/3T3 cell lysate, C6 cell lysate, PC-12 cell lysate, human lung tissue lysate, mouse testis tissue lysate, rat testis tissue lysate, PC-12, MCF7, RAW264.7. |
| Subcellular location: | Cytoplasm. Endosome. Golgi apparatus. Nucleus. |
| Recommended Dilutions:
WB IF-Cell FC |
1:2,000 1:100 1:1,000 |
| Uniprot #: | SwissProt: P62993 Human | Q60631 Mouse | P62994 Rat |
| Alternative names: | Abundant SRC homology Adapter protein GRB2 ASH Ash protein EGFRBP GRB2 Epidermal growth factor receptor binding protein Epidermal growth factor receptor binding protein GRB2 GRB 2 GRB2 adapter protein Grb2 GRB2_HUMAN Grb3 3 Growth factor receptor bound protein 2 Growth factor receptor bound protein 3 Growth factor receptor-bound protein 2 HT027 MST084 MSTP084 NCKAP2 OTTHUMP00000166096 OTTHUMP00000166097 OTTHUMP00000166098 Protein ash SEM5 SH2/SH3 adapter GRB2 |
Images
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Fig1:
Western blot analysis of GRB2 on different lysates with Rabbit anti-GRB2 antibody (HA721923) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: 293T cell lysate Lane 3: A431 cell lysate Lane 4: MCF7 cell lysate Lane 5: HCT 116 cell lysate Lane 6: COS-1 cell lysate Lane 7: RAW264.7 cell lysate Lane 8: NIH/3T3 cell lysate Lane 9: C6 cell lysate Lane 10: PC-12 cell lysate Lane 11: Human lung tissue lysate Lane 12: Mouse testis tissue lysate Lane 13: Rat testis tissue lysate Lysates/proteins at 30 µg/Lane. Predicted band size: 25 kDa Observed band size: 25 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721923) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunocytochemistry analysis of PC-12 cells labeling GRB2 with Rabbit anti-GRB2 antibody (HA721923) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-GRB2 antibody (HA721923) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. |
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Fig3:
Immunocytochemistry analysis of MCF7 cells labeling GRB2 with Rabbit anti-GRB2 antibody (HA721923) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-GRB2 antibody (HA721923) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
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Fig4:
Immunocytochemistry analysis of RAW264.7 cells labeling GRB2 with Rabbit anti-GRB2 antibody (HA721923) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-GRB2 antibody (HA721923) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
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Fig5:
Flow cytometric analysis of MCF7 cells labeling GRB2. Cells were fixed and permeabilized. Then stained with the primary antibody (HA721923, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
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Fig6:
Flow cytometric analysis of PC-12 cells labeling GRB2. Cells were fixed and permeabilized. Then stained with the primary antibody (HA721923, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
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