HLA Class II DRB1 Recombinant Rabbit Monoclonal Antibody [JE60-43]
cat.: HA721927
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P, IF-Tissue |
| Clonality: | Monoclonal |
| Clone number: | JE60-43 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 30 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human HLA Class II DRB1 aa 151-200 / 266. |
| Positive control: | Ramos cell lysate, Raji cell lysate, Daudi cell lysate, HDLM-2 cell lysate, A375 cell lysate, human tonsil tissue, human skin tissue, human diffuse large B-cell lymphoma tissue. |
| Subcellular location: | Cell membrane. Endoplasmic reticulum. |
| Recommended Dilutions:
WB IHC-P IF-Tissue |
1:1,000 1:1,000 1:100 |
| Uniprot #: | SwissProt: P01911 Human | P01912 Human | P04229 Human | P20039 Human | Q29974 Human | Q30134 Human | Q30167 Human | Q5Y7A7 Human | Q95IE3 Human | Q9GIY3 Human |
| Alternative names: | 2B1F_HUMAN DR1 DR16 DR5 DR8 DRB1 DRw10 DW2.2/DR2.2 FLJ75017 FLJ76359 HLA class II antigen beta chain HLA class II histocompatibility antigen, DR-1 beta chain HLA class II histocompatibility antigen, DRB1-15 beta chain HLA-DR-beta 1 HLA-DR1B HLA-DRB HLA-DRB1 human leucocyte antigen DRB1 Leucocyte antigen DR beta 1 chain leucocyte antigen DRB1 lymphocyte antigen DRB1 major histocompatibility complex, class II, DR beta 1 MHC class II antigen MHC class II antigen DRB1*15 MHC class II antigen HLA-DR13 MHC class II HLA-DR beta 1 chain MHC class II HLA-DR-beta cell surface glycoprotein MHC class II HLA-DRw10-beta SS1 |
Images
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Fig1:
Western blot analysis of HLA Class II DRB1 on different lysates with Rabbit anti-HLA Class II DRB1 antibody (HA721927) at 1/1,000 dilution. Lane 1: Ramos cell lysate Lane 2: Raji cell lysate Lane 3: Daudi cell lysate Lane 4: HDLM-2 cell lysate Lane 5: A375 cell lysate Lane 6: HeLa cell lysate (negative) Lysates/proteins at 20 µg/Lane. Predicted band size: 29 kDa Observed band size: 29 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721927) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-HLA Class II DRB1 antibody (HA721927) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721927) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human skin tissue with Rabbit anti-HLA Class II DRB1 antibody (HA721927) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721927) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded human diffuse large B-cell lymphoma tissue with Rabbit anti-HLA Class II DRB1 antibody (HA721927) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721927) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Immunofluorescence analysis of paraffin-embedded human skin tissue labeling HLA Class II DRB1 with Rabbit anti-HLA Class II DRB1 antibody (HA721927) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA721927, green) at 1/100 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue). |
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Fig6:
Immunofluorescence analysis of paraffin-embedded human diffuse large B-cell lymphoma tissue labeling HLA Class II DRB1 with Rabbit anti-HLA Class II DRB1 antibody (HA721927) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA721927, green) at 1/100 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue). |
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