PHD3 Recombinant Rabbit Monoclonal Antibody [JE60-82]
cat.: HA721929
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JE60-82 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 27 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant full length protein within human PHD3. |
| Positive control: | Mouse liver tissue lysate, K-562 cell lysate, A375 cell lysate, HEK-293 cell lysate. |
| Subcellular location: | Cytoplasm. Nucleus. |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: Q9H6Z9 Human | Q91UZ4 Mouse |
| Alternative names: | Egl 9 family hypoxia inducible factor 3 Egl nine homolog 3 (C. elegans) Egl nine homolog 3 Egl nine like protein 3 isoform EGL9 homolog of C. elegans 3 EGLN3 EGLN3_HUMAN Factor responsive smooth muscle protein HIF Prolyl Hydroxylase 3 HIF-PH3 HIF-prolyl hydroxylase 3 HIFP4H3 HIFPH3 HPH-1 HPH-3 Hypoxia-inducible factor prolyl hydroxylase 3 P4H3 PHD3 Prolyl Hydroxylase Domain Containing Protein 3 Prolyl hydroxylase domain-containing protein 3 SM20 |
Images
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Fig1:
Western blot analysis of PHD3 on different lysates with Rabbit anti-PHD3 antibody (HA721929) at 1/1,000 dilution. Lane 1: Mouse liver tissue lysate (40 µg/Lane) Lane 2: K-562 cell lysate (20 µg/Lane) Lane 3: A375 cell lysate (20 µg/Lane) Lane 4: HEK-293 cell lysate (20 µg/Lane) Predicted band size: 27 kDa Observed band size: 30 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721929) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.