CD32A + CD32B + CD32C Recombinant Rabbit Monoclonal Antibody [JE64-17]
cat.: HA721930
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JE64-17 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 35 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human CD32A aa 60-109 / 317. |
| Positive control: | K-562 cell lysate, Raji cell lysate, HL-60 cell lysate, human lung tissue lysate, human pancreas tissue, human spleen tissue, human tonsil tissue. |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
WB IHC-P |
1:1,000 1:500-1:2,000 |
| Uniprot #: | SwissProt: P12318 Human | P31994 Human | P31995 Human |
| Alternative names: | Low affinity immunoglobulin gamma Fc region receptor II-a Low affinity immunoglobulin gamma Fc region receptor II-b Low affinity immunoglobulin gamma Fc region receptor II-c IgG Fc receptor II-a IgG Fc receptor II-b IgG Fc receptor II-c CDw32 Fc-gamma RII-a (Fc-gamma-RIIa; FcRII-a) Fc-gamma RII-b (Fc-gamma-RIIb; FcRII-b) Fc-gamma RII-c (Fc-gamma-RIIc; FcRII-c) CD32 FCGR2A FCGR2B FCGR2C CD32, FCG2, FCGR2A1, IGFR2 |
Images
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Fig1:
Western blot analysis of CD32A + CD32B + CD32C on different lysates with Rabbit anti-CD32A + CD32B + CD32C antibody (HA721930) at 1/1,000 dilution. Lane 1: K-562 cell lysate Lane 2: Raji cell lysate Lane 3: HL-60 cell lysate Lane 4: Human lung tissue lysate Cell lysates/proteins at 20 µg/Lane. Tissue lysates/proteins at 30 µg/Lane. Predicted band size: 35 kDa Observed band size: 35-42 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721930) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human pancreas tissue with Rabbit anti-CD32A + CD32B + CD32C antibody (HA721930) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721930) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded human spleen tissue with Rabbit anti-CD32A + CD32B + CD32C antibody (HA721930) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721930) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig4:
Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-CD32A + CD32B + CD32C antibody (HA721930) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721930) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.