Human IL-23 Recombinant Rabbit Monoclonal Antibody [PSH03-04]
cat.: HA721934
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Det) |
| Clonality: | Monoclonal |
| Clone number: | PSH03-04 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 20.7 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human IL-23A aa 20-189. |
| Positive control: | Recombinant Human IL-23 protein (HA210909). |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
ELISA(Det) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH03-03] to Human Human IL-23 (Detector) (HA721933) and recombinant Human IL-23 protein (HA210909) as the standard. The reference range value is 37-3000 pg/ml. |
| Uniprot #: | SwissProt: Q9NPF7 Human |
| Alternative names: | IL 23 IL 23 A IL 23 subunit alpha IL 23A IL 23p19 IL-23 subunit alpha IL-23-A IL-23p19 IL12B IL23 Il23a IL23A_HUMAN IL23P19 interleukin 12B Interleukin 23 alpha subunit p19 Interleukin 23 p19 subunit interleukin 23 subunit alpha interleukin 23 subunit p19 interleukin six, G CSF related factor Interleukin-23 subunit alpha Interleukin-23 subunit p19 JKA3 induced upon T cell activation MGC79388 P19 SGRF |
Images
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Fig1:
Sandwich ELISA analysis of human IL-23 matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA721933) diluted in carbonate/bicarbonate buffer, at a concentration of 4 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted human IL-23 protein starting from 3000 pg/ml to 0 pg/ml and detect antibody (HA721934)-Biotin (0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
|
Fig2: Conditioned media was harvested after 16 hours. IL-23 was measured in 100% unstimulated and LPS stimulated THP-1 supernatant. The concentrations of IL-23 were interpolated from the IL-23 standard curves. The mean IL-23 concentration was determined to be 1407 pg/mL in LPS stimulated THP-1 supernatant. There was no detectable signal in unstimulated supernatant. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.