Human IL-33 Recombinant Rabbit Monoclonal Antibody [PSH03-06]
cat.: HA721936
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Det) |
| Clonality: | Monoclonal |
| Clone number: | PSH03-06 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 30.8 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human IL-33 aa 109-270. |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
ELISA(Det) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH03-05] to Human Human IL-33 (Capture) (HA721935) and recombinant standard Human Human IL-33 (HA210539) as the standard. The reference range value is 37-1000 pg/ml. |
| Uniprot #: | SwissProt: O95760 Human |
| Alternative names: | C9orf26 CHROMOSOME 9 OPEN READING FRAME 26 DKFZp586H0523 DVS27 DVS27 related protein IL 1F11 IL 33 IL-1F11 IL-33 IL1F11 IL33 IL33_HUMAN Interleukin 1 family member 11 Interleukin 33 INTERLEUKIN 33 NFHEV Interleukin 33 precursor Interleukin-1 family member 11 Interleukin-33 (109-270) Interleukin33 NF HEV NF-HEV NFEHEV NFHEV Nuclear factor for high endothelial venules Nuclear factor from high endothelial venules OTTHUMP00000021041 RP11 575C20.2 |
Images
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Fig1:
Sandwich ELISA analysis of human IL-33 matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA721935) diluted in carbonate/bicarbonate buffer, at a concentration of 4 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted human IL-33 protein starting from 1000 pg/ml to 0 pg/ml and detect antibody (HA721936)-Biotin (0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2: The concentrations of IL-33 were measured in duplicates, interpolated from the IL-33 standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-33 concentration was determined to be 379 pg/mL in cell culture media. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.