Human IL-22 Recombinant Rabbit Monoclonal Antibody [PSH03-11]
cat.: HA721939
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Cap) |
| Clonality: | Monoclonal |
| Clone number: | PSH03-11 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human IL-22 aa 34-179 (HA210691). |
| Positive control: | Recombinant Human IL-22 protein (HA210691). |
| Subcellular location: | Secreted |
| Recommended Dilutions:
ELISA(Cap) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH03-12] to Human Human IL-22 (Detector) (HA721940) and recombinant standard Human Human IL-22 (HA210691) as the standard. The reference range value is 8.2-2,000 pg/ml. |
| Uniprot #: | SwissProt: Q9GZX6 Human |
| Alternative names: | Cytokine Zcyto18 IL 10 related T cell derived inducible factor IL 22 IL D110 IL TIF IL-10-related T-cell-derived-inducible factor IL-22 IL-TIF IL21 Il22 IL22_HUMAN ILD110 ILTIF Interleukin 10 related T cell derived inducible factor interleukin 21 Interleukin 22 Interleukin-22 MGC79382 MGC79384 TIFa TIFIL 23 TIFIL23 UNQ3099/PRO10096 zcyto18 |
Images
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Fig1:
Sandwich ELISA analysis of human IL-22 matched pair antibodies Capture: HA721939, Mouse CRP Rabbit mAb [PSH03-11] Detector: HA721940, Mouse CRP Rabbit mAb [PSH03-12] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA721939) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human IL-22 protein (HA210691) starting from 2000 pg/ml to 0 pg/ml and detect antibody (HA721940, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2: Conditioned media was harvested after 48 hours. IL-22 was measured in 100% unstimulated and PHA stimulated PBMC supernatant.The concentrations of IL-22 were interpolated from the IL-22 standard curves and corrected for sample dilution.The mean IL-22 concentration was determined to be 79.7 pg/mL in PHA stimulated PBMC supernatant and there was no detectable signal in unstimulated supernatant. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.