Human Prolactin Recombinant Rabbit Monoclonal Antibody [PSH03-14]
cat.: HA721942
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Det) |
| Clonality: | Monoclonal |
| Clone number: | PSH03-14 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 25.9 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human Prolactin aa 29-227. |
| Positive control: | Recombinant standard Human Prolactin (HA210676). |
| Subcellular location: | Secreted |
| Recommended Dilutions:
ELISA(Det) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH03-13] to Human Prolactin (Capture) (HA721941) and recombinant standard Human Prolactin (HA210676) as the standard. The reference range value is 61.73-5000 pg/ml. |
| Uniprot #: | SwissProt: P01236 Human |
| Alternative names: | Decidual prolactin GHA1 Growth hormone A1 Lactogenic hormone Luteotropic hormone Mammotropin PRL Prolactin Prolactin precursor |
Images
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Fig1:
Sandwich ELISA analysis of human Prolactin matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA721941) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted Prolactin protein starting from 5000 pg/ml to 0 pg/ml and detect antibody (HA721942)-Biotin (0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2: The concentrations of Prolactin were interpolated from the Prolactin standard curves and corrected for sample dilution.The Prolactin concentration was determined to be 6,297 pg/mL in serum 20%, 6,367 pg/ml in serum 10%, 5,913 pg/ml in serum 5%, 7,660 pg/ml in serum 2.5%. The mean Prolactin concentration was determined to be 6,559 pg/ml in mixed serum from twenty volunteers. |
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Fig3: The concentrations of Prolactin were interpolated from the Prolactin standard curves and corrected for sample dilution.The mean Prolactin concentration was determined to be 2,995 pg/ml in Daudi extract. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.