AIP Recombinant Rabbit Monoclonal Antibody [PSH03-17]
cat.: HA721945
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | PSH03-17 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 38 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human AIP aa 1-330 / 330. |
| Positive control: | HeLa cell lysate, Jurkat cell lysate, HEK-293 cell lysate, THP-1 cell lysate, HCT 1 cell lysate, MCF7 cell lysate, RAW264.7 cell lysate, NIH/3T3 cell lysate, C6 cell lysate, mouse spleen tissue lysate, rat spleen tissue lysate. |
| Subcellular location: | Cytoplasm. |
| Recommended Dilutions:
WB |
1:2,000 |
| Uniprot #: | SwissProt: O00170 Human | O08915 Mouse | Q5FWY5 Rat |
| Alternative names: | AH receptor interacting protein AH receptor-interacting protein AIP AIP_HUMAN ARA 9 ARA9 Aryl hydrocarbon receptor interacting protein Aryl-hydrocarbon receptor-interacting protein fa03h10 FKBP 16 FKBP 37 FKBP16 FKBP37 HBV X associated protein 2 HBV X associated protein HBV X-associated protein 2 HBVX associated protein Immunophilin homolog ARA 9 Immunophilin homolog ARA9 SMTPHN XAP 2 XAP-2 XAP2 |
Images
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Fig1:
Western blot analysis of AIP on different lysates with Rabbit anti-AIP antibody (HA721945) at 1/2,000 dilution. Lane 1: HeLa cell lysate (20 µg/Lane) Lane 2:Jurkat cell lysate (20 µg/Lane) Lane 3: HEK-293 cell lysate (20 µg/Lane) Lane 4: THP-1 cell lysate (20 µg/Lane) Lane 5: HCT 116 cell lysate (20 µg/Lane) Lane 6: MCF7 cell lysate (20 µg/Lane) Lane 7: RAW264.7 cell lysate (20 µg/Lane) Lane 8: NIH/3T3 cell lysate (20 µg/Lane) Lane 9: C6 cell lysate (20 µg/Lane) Lane 10: Mouse spleen tissue lysate (40 µg/Lane) Lane 11: Rat spleen tissue lysate (40 µg/Lane) Predicted band size: 38 kDa Observed band size: 35 kDa Exposure time: 1 minutes 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721945) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of AIP on different lysates with Rabbit anti-AIP antibody (HA721945) at 1/5,000 dilution. Lane 1: A549-WT cell lysate (10 µg/Lane) Lane 2: A549-KD AIP cell lysate (10 µg/Lane) Predicted band size: 38 kDa Observed band size: 35 kDa Exposure time: 2 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721945) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.