BNP Recombinant Rabbit Monoclonal Antibody [JE30-94]
cat.: HA721946
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JE30-94 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 15 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human BNP aa 61-110 / 134. |
| Positive control: | SW480 cell lysate, U-87 MG cell lysate, U-2 OS cell lysate, mouse brain tissue lysate, rat brain tissue lysate, mouse liver tissue. |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
WB IHC-P |
1:1,000 1:200 |
| Uniprot #: | SwissProt: P16860 Human | P40753 Mouse | P13205 Rat |
| Alternative names: | BNP(1-28) BNP(1-29) BNP(1-30) BNP(1-32) BNP(2-31) BNP(3-29) BNP(3-30) BNP(3-32) BNP(4-27) BNP(4-29) BNP(4-30) BNP(4-31) BNP(4-32) BNP(5-29) BNP(5-31) BNP(5-32) Brain natriuretic peptide 32 Brain type natriuretic peptide Gamma brain natriuretic peptide Natriuretic peptide B Natriuretic peptide precursor B natriuretic peptide, brain type Natriuretic peptides B natriuretic protein NPPB |
Images
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Fig1:
Western blot analysis of BNP on different lysates with Rabbit anti-BNP antibody (HA721946) at 1/1,000 dilution. Lane 1: SW480 cell lysate (20 µg/Lane) Lane 2: U-87 MG cell lysate (20 µg/Lane) Lane 3: U-2 OS cell lysate (20 µg/Lane) Lane 4: Mouse brain tissue lysate (30 µg/Lane) Lane 5: Rat brain tissue lysate (30 µg/Lane) Predicted band size: 15 kDa Observed band size: 37 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721946) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-BNP antibody (HA721946) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721946) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.