ATRIP Recombinant Rabbit Monoclonal Antibody [JE54-71]
cat.: HA721947
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JE54-71 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 86 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human ATRIP aa 171-300 / 791. |
| Positive control: | NIH:OVCAR-3 cell lysate, PC-3M cell lysate, MCF7 cell lysate, HeLa cell lysate, Jurkat cell lysate, mouse testis tissue lysate, rat testis tissue lysate, HeLa, human testis tissue, mouse testis tissue. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB IF-Cell IHC-P |
1:1,000 1:100 1:500 |
| Uniprot #: | SwissProt: Q8WXE1 Human | Q8BMG1 Mouse Entrez Gene: 301014 Rat |
| Alternative names: | AGS1 ATIP ATM and Rad3 related interacting protein ATM and Rad3-related-interacting protein ATR interacting protein ATR-interacting protein Atrip ATRIP_HUMAN DKFZp762J2115 FLJ12343 MGC20625 MGC21482 MGC26740 |
Images
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Fig1:
Western blot analysis of ATRIP on different lysates with Rabbit anti-ATRIP antibody (HA721947) at 1/1,000 dilution. Lane 1: NIH:OVCAR-3 cell lysate (20 µg/Lane) Lane 2: PC-3M cell lysate (20 µg/Lane) Lane 3: MCF7 cell lysate (20 µg/Lane) Lane 4: HeLa cell lysate (20 µg/Lane) Lane 5: Jurkat cell lysate (20 µg/Lane) Lane 6: Mouse testis tissue lysate (30 µg/Lane) Lane 7: Rat testis tissue lysate (30 µg/Lane) Predicted band size: 86 kDa Observed band size: 86 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721947) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunocytochemistry analysis of HeLa cells labeling ATRIP with Rabbit anti-ATRIP antibody (HA721947) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-ATRIP antibody (HA721947) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-ATRIP antibody (HA721947) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721947) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded mouse testis tissue with Rabbit anti-ATRIP antibody (HA721947) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721947) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.