ketohexokinase Recombinant Rabbit Monoclonal Antibody [PSH03-19]
cat.: HA721950
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, FC |
| Clonality: | Monoclonal |
| Clone number: | PSH03-19 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 33 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human ketohexokinase aa 1-298 / 298. |
| Positive control: | HepG2 cell lysate, human liver tissue lysate, human kidney tissue lysate, mouse liver tissue lysate, mouse kidney tissue lysate, rat liver tissue lysate, rat kidney tissue lysate, HepG2. |
| Subcellular location: | Cytoplasm, cytosol, extracellular exosome. |
| Recommended Dilutions:
WB IF-Cell FC |
1:2,000-1:5,000 1:100 1:1,000 |
| Uniprot #: | SwissProt: P50053 Human | P97328 Mouse | Q02974 Rat |
| Alternative names: | EC 2.7.1.3 Fructokinase Hepatic fructokinase Ketohexokinase Ketohexokinase isoform a KHK KHK_HUMAN |
Images
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Fig1:
Western blot analysis of ketohexokinase on different lysates with Rabbit anti-ketohexokinase antibody (HA721950) at 1/2,000 dilution. Lane 1: HepG2 cell lysate Lane 2: Human liver tissue lysate Lane 3: Human kidney tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 33 kDa Observed band size: 33 kDa Exposure time: 11 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721950) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Western blot analysis of ketohexokinase on different lysates with Rabbit anti-ketohexokinase antibody (HA721950) at 1/5,000 dilution. Lane 1: Mouse liver tissue lysate Lane 2: Mouse kidney tissue lysate Lane 3: Rat liver tissue lysate Lane 4: Rat kidney tissue lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 33 kDa Observed band size: 33 kDa Exposure time: 5 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721950) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig3:
Western blot analysis of ketohexokinase on different lysates with Rabbit anti-ketohexokinase antibody (HA721950) at 1/2,000 dilution. Lane 1: A549-si NT cell lysate Lane 2: A549-si ketohexokinase cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 33 kDa Observed band size: 33 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721950) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig4:
Immunocytochemistry analysis of HepG2 cells labeling ketohexokinase with Rabbit anti-ketohexokinase antibody (HA721950) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-ketohexokinase antibody (HA721950) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. |
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Fig5:
Flow cytometric analysis of HepG2 cells labeling ketohexokinase. Cells were fixed and permeabilized. Then stained with the primary antibody (HA721950, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.