Adipose Triglyceride Lipase Recombinant Rabbit Monoclonal Antibody [JE37-49]
cat.: HA721951
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, IF-Tissue |
| Clonality: | Monoclonal |
| Clone number: | JE37-49 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 55 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human Adipose Triglyceride Lipase aa 330-450 / 504. |
| Positive control: | SiHa cell lysate, HepG2 cell lysate, HEK-293 cell lysate, A431 cell lysate, mouse heart tissue lysate, rat heart tissue lysate, mouse brown adipose tissue, rat breast tissue. |
| Subcellular location: | Lipid droplet, Cell membrane, Cytoplasm. |
| Recommended Dilutions:
WB IHC-P IF-Tissue |
1:1,000 1:500-1:2,000 1:200 |
| Uniprot #: | SwissProt: Q96AD5 Human | Q8BJ56 Mouse | P0C548 Rat |
| Alternative names: | 1110001C14Rik Adipose triglyceride lipase ATGL ATGL DESNUTRIN Calcium independent phospholipase A2 Calcium-independent phospholipase A2 Desnutrin EC 3.1.1.3 FP17548 IPLA2 zeta IPLA2-zeta Mutant patatin like phospholipase domain containing 2 Patatin like phospholipase domain containing 2 PATATIN LIKE PHOSPHOLIPASE DOMAIN CONTAINING PROTEIN 2 Patatin-like phospholipase domain-containing protein 2 PEDF R PHOSPHOLIPASE A2 CALCIUM INDEPENDENT ZETA Pigment epithelium derived factor Pigment epithelium-derived factor plpl plpl2 PLPL2_HUMAN Pnpla2 Transport secretion protein 2 Transport secretion protein 2.2 Transport-secretion protein 2 Triglyceride hydrolase TTS 2.2 TTS2 TTS2.2 ZETA |
Images
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Fig1:
Western blot analysis of Adipose Triglyceride Lipase on different lysates with Rabbit anti-Adipose Triglyceride Lipase antibody (HA721951) at 1/1,000 dilution. Lane 1: SiHa cell lysate (30 µg/Lane) Lane 2: HepG2 cell lysate (30 µg/Lane) Lane 3: HEK-293 cell lysate (30 µg/Lane) Lane 4: A431 cell lysate (30 µg/Lane) Lane 5: Mouse heart tissue lysate (40 µg/Lane) Lane 6: Rat heart tissue lysate (40 µg/Lane) Predicted band size: 55 kDa Observed band size: 55 kDa Exposure time: Lane 1-4: 3 minutes; Lane 5-6: 5 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721951) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded mouse brown adipose tissue with Rabbit anti-Adipose Triglyceride Lipase antibody (HA721951) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721951) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded rat breast tissue with Rabbit anti-Adipose Triglyceride Lipase antibody (HA721951) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721951) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig4:
Immunofluorescence analysis of paraffin-embedded mouse brown adipose tissue labeling Adipose Triglyceride Lipase with Rabbit anti-Adipose Triglyceride Lipase antibody (HA721951) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA721951, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.