IFNGR1 Recombinant Rabbit Monoclonal Antibody [JE40-50]
cat.: HA721952
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IHC-P
Clonality: Monoclonal
Clone number: JE40-50
Form: Liquid
Storage condition: Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 54 kDa
Isotype: IgG
Immunogen: Recombinant protein within Human IFNGR1 aa 390-489 / 489.
Positive control: NCI-H226 cell lysate, LoVo cell lysate, HepG2 cell lysate, THP-1 cell lysate, K-562 cell lysate, HEK-293 cell lysate, HL-60 cell lysate, SW620 cell lysate, mouse liver tissue lysate, mouse thymus tissue lysate, human kidney tissue, human placenta tissue.
Subcellular location: Cell membrane.
Recommended Dilutions:
  WB
  IHC-P
1:1,000-1:2,000
1:2,000
Uniprot #: SwissProt: P15260 Human | P15261 Mouse | Q9QZ62 Rat
Alternative names: Antiviral Protein Type II Antiviral protein, type 2 AVP type II AVP, type 2 CD 119 CD119 CD119 antigen CDw119 FLJ45734 IFN gamma R alpha IFN gamma R IFN gamma R1 IFN-gamma receptor 1 IFN-gamma-R1 IFNG R1 IFNGR 1 IFNGR IFNGR1 Immune interferon receptor 1 Immune interferon receptor for INGR1_HUMAN Interferon gamma receptor 1 Interferon gamma receptor alpha chain Interferon gamma receptor alpha chain precursor
Images
HA721952_1.jpg Fig1: Western blot analysis of IFNGR1 on different lysates with Rabbit anti-IFNGR1 antibody (HA721952) at 1/1,000 dilution.

Lane 1: NCI-H226 cell lysate (20 µg/Lane)
Lane 2: LoVo cell lysate (20 µg/Lane)
Lane 3: HepG2 cell lysate (20 µg/Lane)
Lane 4: THP-1 cell lysate (20 µg/Lane)
Lane 5: K-562 cell lysate (20 µg/Lane)
Lane 6: HEK-293 cell lysate (20 µg/Lane)
Lane 7: MCF7 cell lysate (low expression) (20 µg/Lane)
Lane 8: HL-60 cell lysate (20 µg/Lane)
Lane 9: SW620 cell lysate (20 µg/Lane)
Lane 10: Mouse liver tissue lysate (40 µg/Lane)
Lane 11: Mouse thymus tissue lysate (40 µg/Lane)

Predicted band size: 54 kDa
Observed band size: 40-100 kDa

Exposure time: 1 minute 2 seconds; ECL: K1801;
4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721952) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
HA721952_2.jpg Fig2: Western blot analysis of IFNGR1 on different lysates with Rabbit anti-IFNGR1 antibody (HA721952) at 1/2,000 dilution.

Lane 1: HAP1-parental cell lysate
Lane 2: HAP1-IFNGR1 KD cell lysate

Lysates/proteins at 10 µg/Lane.

Predicted band size: 54 kDa
Observed band size: 40-100 kDa

Exposure time: 180 seconds; ECL: K1801;
4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721952) at 1/2,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
HA721952_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-IFNGR1 antibody (HA721952) at 1/2,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721952) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA721952_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human placenta tissue with Rabbit anti-IFNGR1 antibody (HA721952) at 1/2,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721952) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA721952_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human tonsil
tissue with Rabbit anti-IFNGR1 antibody (HA721952) at 1/2,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721952) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA721952_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded mouse kidney
tissue with Rabbit anti-IFNGR1 antibody (HA721952) at 1/2,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721952) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA721952_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded rat spleen
tissue with Rabbit anti-IFNGR1 antibody (HA721952) at 1/2,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721952) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.