3mC Recombinant Rabbit Monoclonal Antibody [PSH03-21]
cat.: HA721957
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Species independent |
| Applications: | IF-Cell, Dot Blot |
| Clonality: | Monoclonal |
| Clone number: | PSH03-21 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | 3-mc-OVA |
| Positive control: | U-2 OS cells treated with 4mM Methyl Methanesulfonate for 1 hour. |
| Recommended Dilutions:
IF-Cell Dot Blot |
1:100 1:2,000 |
Images
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Fig1:
Immunocytochemistry analysis of U-2 OS cells treated with 4mM Methyl Methanesulfonate for 1 hour labeling 3mC with Rabbit anti-3mC antibody (HA721957) at 1/100 dilution. Cells were fixed in 70% ethyl alcohol for 5 minutes at room temperature, then subjected to acid hydrolysis using 2M HCl in TBST for 30 minutes at room temperature. Permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 2% BSA for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-3mC antibody (HA721957) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. |
|
Fig2:
Dot blot analysis of 3mC on different proteins with Rabbit anti-3mC antibody (HA721957) at 1/2,000 dilution. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution for 1 hour at room temperature. Lane 1: Uridine-BSA (negative) Lane 2: Cytidine-BSA (negative) Lane 3: 5-Methylcytosine-BSA (negative) Lane 4: 3-Methylcytosine-BSA (positive) Lane 5: 5-Hydroxymethylcytidine-BSA (negative) Proteins loading: 50ng, 25ng, 12.5ng, 6.25ng; Blocking and dilution buffer: 5% NFDM/TBST; Exposure time: 1 minute. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.