TRPM8 Recombinant Rabbit Monoclonal Antibody [JE32-58]
cat.: HA721959
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JE32-58 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 128 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human TRPM8 aa 851-1,050 / 1,104. |
| Positive control: | LNCaP cell lysate, MCF7 cell lysate, HCT 116 cell lysate, human liver cancer tissue, human prostate cancer tissue. |
| Subcellular location: | Cell membrane, Membrane raft, Endoplasmic reticulum membrane. |
| Recommended Dilutions:
WB IHC-P |
1:1,000 1:500 |
| Uniprot #: | SwissProt: Q7Z2W7 Human |
| Alternative names: | Long transient receptor potential channel 6 LTrpC-6 LTrpC6 MGC2849 Short form of the TRPM8 cationic channel Transient receptor potential cation channel subfamily M member 8 Transient receptor potential p8 transient receptor potential-p8 Trp p8 Trp-p8 Trpm8 TRPM8_HUMAN TRPP8 |
Images
|
Fig1:
Western blot analysis of TRPM8 on different lysates with Rabbit anti-TRPM8 antibody (HA721959) at 1/1,000 dilution. Lane 1: LNCaP cell lysate Lane 2: MCF7 cell lysate Lane 3: HCT 116 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 128 kDa Observed band size: 110 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721959) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue with Rabbit anti-TRPM8 antibody (HA721959) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721959) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue with Rabbit anti-TRPM8 antibody (HA721959) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721959) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.