p18 INK4c Recombinant Rabbit Monoclonal Antibody [JE36-09]
cat.: HA721960
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JE36-09 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 18 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human p18 INK4c aa 1-150 / 168. |
| Positive control: | HeLa cell lysate, 293T cell lysate, MCF7 cell lysate, A549 cell lysate, mouse skeletal muscle tissue lysate, rat skeletal muscle tissue lysate, human skeletal muscle tissue. |
| Subcellular location: | Cytoplasm, cytosol, nucleus. |
| Recommended Dilutions:
WB IHC-P |
1:1,000 1:500 |
| Uniprot #: | SwissProt: P42773 Human | Q60772 Mouse Entrez Gene: 54238 Rat |
| Alternative names: | CDK6 inhibitor p18 CDKN 2C CDKN 6 Cdkn2c CDKN6 CDN2C_HUMAN Cyclin dependent inhibitor Cyclin dependent kinase 4 inhibitor C Cyclin dependent kinase 6 inhibitor Cyclin dependent kinase 6 inhibitor p18 Cyclin dependent kinase inhibitor 2C (p18 inhibits CDK4) Cyclin dependent kinase inhibitor 2C Cyclin-dependent kinase 4 inhibitor C Cyclin-dependent kinase 6 inhibitor INK4C OTTHUMP00000046546 p18 p18 inhibits CDK4 p18 INK4c p18 INK6 p18-INK4c p18-INK6 |
Images
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Fig1:
Western blot analysis of p18 INK4c on different lysates with Rabbit anti-p18 INK4c antibody (HA721960) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 µg/Lane) Lane 2: 293T cell lysate (20 µg/Lane) Lane 3: MCF7 cell lysate (20 µg/Lane) Lane 4: A549 cell lysate (20 µg/Lane) Lane 5: Mouse skeletal muscle tissue lysate (40 µg/Lane) Lane 6: Rat skeletal muscle tissue lysate (40 µg/Lane) Predicted band size: 18 kDa Observed band size: 18 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721960) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue with Rabbit anti-p18 INK4c antibody (HA721960) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721960) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.