PTEN Recombinant Rabbit Monoclonal Antibody [JE51-96]
cat.: HA721962
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IHC-P, FC
Clonality: Monoclonal
Clone number: JE51-96
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 47 kDa
Isotype: IgG
Immunogen: Recombinant full length protein of Human PTEN.
Positive control: HeLa cell lysate, MCF7 cell lysate, A431 cell lysate, NIH/3T3 cell lysate, C6 cell lysate, PC-12 cell lysate, mouse brain tissue, rat brain tissue, A431, C6.
Subcellular location: Cytoplasm, Nucleus, Secreted.
Recommended Dilutions:
  WB
  IHC-P
  FC

1:1,000
1:500
1:1,000
Uniprot #: SwissProt: P60484 Human | O08586 Mouse
Unigene: 22158 Rat
Alternative names: 10q23del BZS DEC GLM2 MGC11227 MHAM MMAC1 MMAC1 phosphatase and tensin homolog deleted on chromosome 10 Mutated in multiple advanced cancers 1 Phosphatase and tensin homolog Phosphatase and tensin like protein Phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN Pten PTEN_HUMAN PTEN1 TEP1
Images
HA721962_1.jpg Fig1: Western blot analysis of PTEN on different lysates with Rabbit anti-PTEN antibody (HA721962) at 1/1,000 dilution.

Lane 1: HeLa cell lysate
Lane 2: MCF7 cell lysate
Lane 3: MDA-MB-468 cell lysate (negative)
Lane 4: A431 cell lysate
Lane 5: NIH/3T3 cell lysate
Lane 6: C6 cell lysate
Lane 7: PC-12 cell lysate

Lysates/proteins at 20 µg/Lane.

Predicted band size: 47 kDa
Observed band size: 55 kDa

Exposure time: 2 minutes 18 seconds; ECL: K1802;
4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721962) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
HA721962_2.jpg Fig2: Western blot analysis of PTEN on different lysates with Rabbit anti-PTEN antibody (HA721962) at 1/1,000 dilution.

Lane 1: A549-si NT cell lysate
Lane 2: A549-si PTEN cell lysate

Lysates/proteins at 20 µg/Lane.

Predicted band size: 47 kDa
Observed band size: 55 kDa

Exposure time: 45 seconds; ECL: K1801;
4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721962) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
HA721962_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-PTEN antibody (HA721962) at 1/500 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721962) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA721962_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-PTEN antibody (HA721962) at 1/500 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721962) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA721962_5.jpg Fig5: Flow cytometric analysis of A431 cells labeling PTEN.

Cells were fixed and permeabilized. Then stained with the primary antibody (HA721962, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
HA721962_6.jpg Fig6: Flow cytometric analysis of C6 cells labeling PTEN.

Cells were fixed and permeabilized. Then stained with the primary antibody (HA721962, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.