KSR1 Recombinant Rabbit Monoclonal Antibody [JE57-75]
cat.: HA721963
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, FC |
| Clonality: | Monoclonal |
| Clone number: | JE57-75 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 102 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human KSR1 aa 874-923 / 923. |
| Positive control: | 293T cell lysate, HeLa cell lysate, HT-29 cell lysate, HDLM-2 cell lysate, C2C12 cell lysate, C6 cell lysate, human cervical carcinoma tissue, C6. |
| Subcellular location: | Cytoplasm, Membrane, Cell membrane, Cell projection, ruffle membrane, Endoplasmic reticulum membrane. |
| Recommended Dilutions:
WB IHC-P FC |
1:1,000 1:200 1:1,000 |
| Uniprot #: | SwissProt: Q8IVT5 Human | Q61097 Mouse Entrez Gene: 360573 Rat |
| Alternative names: | BKSR 1 BKSR1 dKsr EK 31 EK31 hb Kinase suppressor of Ras 1 Kinase suppressor of ras KSR 1 KSR KSR1 KSR1_HUMAN Positive Ras signaling mediator family member (86.4 kD) Positive Ras signaling mediator family member RSU 2 RSU2 SR 31 SR31 Suppressor of activated let 60 Ras SUR3 Suppressor of Ras1 31 |
Images
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Fig1:
Western blot analysis of KSR1 on different lysates with Rabbit anti-KSR1 antibody (HA721963) at 1/1,000 dilution. Lane 1: 293T cell lysate Lane 2: HeLa cell lysate Lane 3: HT-29 cell lysate Lane 4: HDLM-2 cell lysate Lane 5: C2C12 cell lysate Lane 6: C6 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 102 kDa Observed band size: 120/100 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721963) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human cervical carcinoma tissue with Rabbit anti-KSR1 antibody (HA721963) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721963) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Flow cytometric analysis of C6 cells labeling KSR1. Cells were fixed and permeabilized. Then stained with the primary antibody (HA721963, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.