PCTAIRE1 Recombinant Rabbit Monoclonal Antibody [JE64-55]
cat.: HA721964
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB
Clonality: Monoclonal
Clone number: JE64-55
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 56 kDa
Isotype: IgG
Immunogen: Recombinant protein within Human PCTAIRE1 aa 1-150 / 496.
Positive control: HepG2 cell lysate, Neuro-2a cell lysate, SH-SY5Y cell lysate, U-87 MG cell lysate, mouse brain tissue lysate, rat brain tissue lysate, rat pancreas tissue lysate.
Subcellular location: Cytoplasm, Cytoplasmic vesicle, secretory vesicle, Cell membrane, Synapse, synaptosome.
Recommended Dilutions:
  WB

1:2,000
Uniprot #: SwissProt: Q00536 Human | Q04735 Mouse | Q63686 Rat
Alternative names: Cdk16 CDK16_HUMAN Cell division protein kinase 16 Cyclin-dependent kinase 16 PCTAIRE PCTAIRE protein kinase 1 PCTAIRE-motif protein kinase 1 PCTAIRE1 PCTGAIRE PCTK1 Serine/threonine-protein kinase PCTAIRE-1
Images
HA721964_1.jpg Fig1: Western blot analysis of PCTAIRE1 on different lysates with Rabbit anti-PCTAIRE1 antibody (HA721964) at 1/2,000 dilution.

Lane 1: HepG2 cell lysate
Lane 2: Neuro-2a cell lysate
Lane 3: SH-SY5Y cell lysate
Lane 4: U-87 MG cell lysate
Lane 5: Mouse brain tissue lysate
Lane 6: Rat brain tissue lysate
Lane 7: Rat pancreas tissue lysate

Lysates/proteins at 20 µg/Lane.

Predicted band size: 56 kDa
Observed band size: 56/63 kDa

Exposure time: 2 minutes;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721964) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.