Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
---|---|
Species reactivity: | Human, Mouse, Rat |
Applications: | WB |
Clonality: | Monoclonal |
Clone number: | JE64-55 |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 1ug/ul |
Purification: | Protein A affinity purified. |
Molecular weight: | Predicted band size: 56 kDa |
Isotype: | IgG |
Immunogen: | Recombinant protein within Human PCTAIRE1 aa 1-150 / 496. |
Positive control: | HepG2 cell lysate, Neuro-2a cell lysate, SH-SY5Y cell lysate, U-87 MG cell lysate, mouse brain tissue lysate, rat brain tissue lysate, rat pancreas tissue lysate. |
Subcellular location: | Cytoplasm, Cytoplasmic vesicle, secretory vesicle, Cell membrane, Synapse, synaptosome. |
Recommended Dilutions:
WB |
1:2,000 |
Uniprot #: | SwissProt: Q00536 Human | Q04735 Mouse | Q63686 Rat |
Alternative names: | Cdk16 CDK16_HUMAN Cell division protein kinase 16 Cyclin-dependent kinase 16 PCTAIRE PCTAIRE protein kinase 1 PCTAIRE-motif protein kinase 1 PCTAIRE1 PCTGAIRE PCTK1 Serine/threonine-protein kinase PCTAIRE-1 |
Fig1:
Western blot analysis of PCTAIRE1 on different lysates with Rabbit anti-PCTAIRE1 antibody (HA721964) at 1/2,000 dilution. Lane 1: HepG2 cell lysate Lane 2: Neuro-2a cell lysate Lane 3: SH-SY5Y cell lysate Lane 4: U-87 MG cell lysate Lane 5: Mouse brain tissue lysate Lane 6: Rat brain tissue lysate Lane 7: Rat pancreas tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 56 kDa Observed band size: 56/63 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721964) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |