Ninein Recombinant Rabbit Monoclonal Antibody [PSH03-23]
cat.: HA721965
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | PSH03-23 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 243 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human Ninein aa 1-500 / 2,090. |
| Positive control: | HeLa cell lysate, A549 cell lysate, HT-29 cell lysate, MEF cell lysate, human lung tissue lysate, mouse lung tissue lysate. |
| Subcellular location: | Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole. |
| Recommended Dilutions:
WB |
1:2,000 |
| Uniprot #: | SwissProt: Q8N4C6 Human | Q61043 Mouse |
| Alternative names: | Centrosome Marker Glycogen synthase kinase 3 beta interacting protein GSK3B interacting protein h Glycogen synthase kinase 3 beta-interacting protein hNinein KIAA1565 NIN NIN protein NIN_HUMAN ninein (GSK3B interacting protein) Ninein Ninein centrosomal protein SCKL7 |
Images
|
Fig1:
Western blot analysis of Ninein on different lysates with Rabbit anti-Ninein antibody (HA721965) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: A549 cell lysate Lane 3: HT-29 cell lysate Lane 4: MEF cell lysate Lane 5: Human lung tissue lysate Lane 6: Mouse lung tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 243 kDa Observed band size: 270 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721965) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.