RAB10 Recombinant Rabbit Monoclonal Antibody [PSH03-24]
cat.: HA721966
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | PSH03-24 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 23 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human RAB10 aa 101-150 / 200. |
| Positive control: | A549 cell lysate, HeLa cell lysate, MCF7 cell lysate, HCT 116 cell lysate, COS-1 cell lysate, NIH/3T3 cell lysate, C6 cell lysate, mouse brain tissue lysate, rat brain tissue lysate. |
| Subcellular location: | Cytoplasmic vesicle membrane. |
| Recommended Dilutions:
WB |
1:1,000-1:2,000 |
| Uniprot #: | SwissProt: P61026 Human | P61027 Mouse | P35281 Rat |
| Alternative names: | GTP binding protein RAB10 Rab10 RAB10 member RAS oncogene family RAB10_HUMAN Ras related GTP binding protein Ras related GTP binding protein RAB10 Ras-related protein Rab-10 |
Images
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Fig1:
Western blot analysis of RAB10 on different lysates with Rabbit anti-RAB10 antibody (HA721966) at 1/2,000 dilution. Lane 1: A549 cell lysate Lane 2: HeLa cell lysate Lane 3: MCF7 cell lysate Lane 4: HCT 116 cell lysate Lane 5: COS-1 cell lysate Lane 6: NIH/3T3 cell lysate Lane 7: C6 cell lysate Lane 8: Mouse brain tissue lysate Lane 9: Rat brain tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 23 kDa Observed band size: 23 kDa Exposure time: 5 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721966) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of RAB10 on different lysates with Rabbit anti-RAB10 antibody (HA721966) at 1/1,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-RAB10 KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 23 kDa Observed band size: 23 kDa Exposure time: 180 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721966) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.