FMO3 Recombinant Rabbit Monoclonal Antibody [JE55-13]
cat.: HA721971
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JE55-13 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 60 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human FMO3 aa 281-380 / 532. |
| Positive control: | Human kidney tissue lysate, mouse liver tissue lysate, mouse kidney tissue lysate, rat liver tissue lysate, rat kidney tissue lysate, human liver tissue, mouse liver tissue, rat liver tissue. |
| Subcellular location: | Microsome membrane, Endoplasmic reticulum membrane. |
| Recommended Dilutions:
WB IHC-P |
1:1,000 1:500-1:2,000 |
| Uniprot #: | SwissProt: P31513 Human | P97501 Mouse | Q9EQ76 Rat |
| Alternative names: | Dimethylaniline monooxygenase [N oxide forming] 3 Dimethylaniline monooxygenase [N-oxide-forming] 3 Dimethylaniline monooxygenase 3 Dimethylaniline oxidase 3 dJ127D3.1 Flavin containing monooxygenase 3 FMO 3 FMO form 2 FMO II FMO3 FMO3_HUMAN FMOII Hepatic flavin containing monooxygenase 3 Hepatic flavin-containing monooxygenase 3 MGC34400 TMAU Trimethylamine monooxygenase |
Images
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Fig1:
Western blot analysis of FMO3 on different lysates with Rabbit anti-FMO3 antibody (HA721971) at 1/1,000 dilution. Lane 1: Human kidney tissue lysate Lane 2: Mouse liver tissue lysate Lane 3: Mouse kidney tissue lysate Lane 4: Rat liver tissue lysate Lane 5: Rat kidney tissue lysate Lysates/proteins at 30 µg/Lane. Predicted band size: 60 kDa Observed band size: 56 kDa Exposure time: 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721971) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-FMO3 antibody (HA721971) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721971) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-FMO3 antibody (HA721971) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721971) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig4:
Immunohistochemical analysis of paraffin-embedded rat liver tissue with Rabbit anti-FMO3 antibody (HA721971) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721971) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.