IQGAP1 Recombinant Rabbit Monoclonal Antibody [JE50-66]
cat.: HA721972
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IF-Cell, IHC-P, FC
Clonality: Monoclonal
Clone number: JE50-66
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size:
Isotype: IgG
Immunogen: Synthetic peptide within Human IQGAP1 aa 1-50 / 1,657.
Positive control: HeLa cell lysate, 293T cell lysate, MDA-MB-231 cell lysate, RAW264.7 cell lysate, NIH/3T3 cell lysate, C6 cell lysate, HeLa, human liver tissue.
Subcellular location: Cell membrane, Nucleus, Cytoplasm, Apical cell membrane, Basolateral cell membrane.
Recommended Dilutions:
  WB
  IF-Cell
  IHC-P
  FC
1:2,000
1:100
1:4,000
1:1,000
Uniprot #: SwissProt: P46940 Human | Q9JKF1 Mouse | B5DFH1 Rat
Alternative names: HUMORFA01 IQ motif containing GTPase activating protein 1 IQGA1_HUMAN Iqgap1 KIAA0051 p195 Ras GTPase activating like protein 1 Ras GTPase-activating-like protein IQGAP1 RasGAP-like with IQ motifs SAR1
Images
HA721972_1.jpg Fig1: Western blot analysis of IQGAP1 on different lysates with Rabbit anti-IQGAP1 antibody (HA721972) at 1/2,000 dilution.

Lane 1: HeLa cell lysate
Lane 2: 293T cell lysate
Lane 3: MDA-MB-231 cell lysate
Lane 4: RAW264.7 cell lysate
Lane 5: NIH/3T3 cell lysate
Lane 6: C6 cell lysate

Lysates/proteins at 20 µg/Lane.

Predicted band size: 189 kDa
Observed band size: 189 kDa

Exposure time: 1 minute 40 second;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721972) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
HA721972_2.jpg Fig2: Immunocytochemistry analysis of HeLa cells labeling IQGAP1 with Rabbit anti-IQGAP1 antibody (HA721972) at 1/100 dilution.

Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-IQGAP1 antibody (HA721972) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
HA721972_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-IQGAP1 antibody (HA721972) at 1/4,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721972) at 1/4,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA721972_4.jpg Fig4: Flow cytometric analysis of HeLa cells labeling IQGAP1.

Cells were fixed and permeabilized. Then stained with the primary antibody (HA721972, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.