AER61 Recombinant Rabbit Monoclonal Antibody [JE64-87]
cat.: HA721973
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JE64-87 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 62 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human AER61 aa 1-200 / 527. |
| Positive control: | Neuro-2a cell lysate, U-87 MG cell lysate, HeLa cell lysate, Caco-2 cell lysate, HDLM-2 cell lysate, HepG2 cell lysate, mouse brain tissue lysate, RAW264.7 cell lysate, mouse heart tissue lysate, mouse kidney tissue lysate, rat kidney tissue lysate. |
| Subcellular location: | Endoplasmic reticulum lumen. |
| Recommended Dilutions:
WB |
1:2,000 |
| Uniprot #: | SwissProt: Q5NDL2 Human | Q8BYW9 Mouse | Q5NDL0 Rat |
| Alternative names: | AER61 glycosyltransferase AOS4 C3orf64 EGF domain specific O linked N acetylglucosamine transferase EGF domain-specific O-linked N-acetylglucosamine transferase EGF O GlcNAc transferase EGF-O-GlcNAc transferase EOGT EOGT_HUMAN EOGT1 Extracellular O-linked N-acetylglucosamine transferase Uncharacterized glycosyltransferase AER61 |
Images
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Fig1:
Western blot analysis of AER61 on different lysates with Rabbit anti-AER61 antibody (HA721973) at 1/2,000 dilution. Lane 1: Neuro-2a cell lysate (20 µg/Lane) Lane 2: U-87 MG cell lysate (20 µg/Lane) Lane 3: HeLa cell lysate (20 µg/Lane) Lane 4: Caco-2 cell lysate (20 µg/Lane) Lane 5: HDLM-2 cell lysate (20 µg/Lane) Lane 6: HepG2 cell lysate (20 µg/Lane) Lane 7: Mouse brain tissue lysate (40 µg/Lane) Predicted band size: 62 kDa Observed band size: 62 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721973) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of AER61 on different lysates with Rabbit anti-AER61 antibody (HA721973) at 1/2,000 dilution. Lane 1: RAW264.7 cell lysate (20 µg/Lane) Lane 2: Mouse heart tissue lysate (40 µg/Lane) Lane 3: Mouse kidney tissue lysate (40 µg/Lane) Lane 4: Rat kidney tissue lysate (40 µg/Lane) Predicted band size: 62 kDa Observed band size: 62 kDa Exposure time: 1 minute 59 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721973) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.