AER61 Recombinant Rabbit Monoclonal Antibody [JE64-87]
cat.: HA721973
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB
Clonality: Monoclonal
Clone number: JE64-87
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 62 kDa
Isotype: IgG
Immunogen: Recombinant protein within Human AER61 aa 1-200 / 527.
Positive control: Neuro-2a cell lysate, U-87 MG cell lysate, HeLa cell lysate, Caco-2 cell lysate, HDLM-2 cell lysate, HepG2 cell lysate, mouse brain tissue lysate, RAW264.7 cell lysate, mouse heart tissue lysate, mouse kidney tissue lysate, rat kidney tissue lysate.
Subcellular location: Endoplasmic reticulum lumen.
Recommended Dilutions:
  WB

1:2,000
Uniprot #: SwissProt: Q5NDL2 Human | Q8BYW9 Mouse | Q5NDL0 Rat
Alternative names: AER61 glycosyltransferase AOS4 C3orf64 EGF domain specific O linked N acetylglucosamine transferase EGF domain-specific O-linked N-acetylglucosamine transferase EGF O GlcNAc transferase EGF-O-GlcNAc transferase EOGT EOGT_HUMAN EOGT1 Extracellular O-linked N-acetylglucosamine transferase Uncharacterized glycosyltransferase AER61
Images
HA721973_1.jpg Fig1: Western blot analysis of AER61 on different lysates with Rabbit anti-AER61 antibody (HA721973) at 1/2,000 dilution.

Lane 1: Neuro-2a cell lysate (20 µg/Lane)
Lane 2: U-87 MG cell lysate (20 µg/Lane)
Lane 3: HeLa cell lysate (20 µg/Lane)
Lane 4: Caco-2 cell lysate (20 µg/Lane)
Lane 5: HDLM-2 cell lysate (20 µg/Lane)
Lane 6: HepG2 cell lysate (20 µg/Lane)
Lane 7: Mouse brain tissue lysate (40 µg/Lane)

Predicted band size: 62 kDa
Observed band size: 62 kDa

Exposure time: 3 minutes;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721973) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
HA721973_2.jpg Fig2: Western blot analysis of AER61 on different lysates with Rabbit anti-AER61 antibody (HA721973) at 1/2,000 dilution.

Lane 1: RAW264.7 cell lysate (20 µg/Lane)
Lane 2: Mouse heart tissue lysate (40 µg/Lane)
Lane 3: Mouse kidney tissue lysate (40 µg/Lane)
Lane 4: Rat kidney tissue lysate (40 µg/Lane)

Predicted band size: 62 kDa
Observed band size: 62 kDa

Exposure time: 1 minute 59 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721973) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.