Mouse IL-1 beta Recombinant Rabbit Monoclonal Antibody [PSH03-31]
cat.: HA721976
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse |
| Applications: | ELISA(Cap) |
| Clonality: | Monoclonal |
| Clone number: | PSH03-31 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 30.9 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Mouse IL-1 beta aa 118-269 (P10749). |
| Positive control: | Recombinant Mouse IL-1 beta protein. |
| Subcellular location: | Secreted |
| Recommended Dilutions:
ELISA(Cap) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH03-32] to Mouse IL1 beta (Dctector) (HA721977) and recombinant standard Mouse IL1 beta protein (HA210706) as the standard. The reference range value is 37-3000pg/ml. |
| Uniprot #: | SwissProt: P10749 Mouse |
| Alternative names: | Catabolin H1 IFN beta inducing factor IL 1 IL 1 beta IL-1 beta IL1 IL1 BETA IL1B IL1B_HUMAN IL1F2 Interleukin 1 beta Interleukin 1 beta precursor interleukin 1, beta Interleukin-1 beta OAF Osteoclast activating factor OTTHUMP00000162031 Preinterleukin 1 beta Preinterleukin beta Pro interleukin 1 beta |
Images
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Fig1:
Sandwich ELISA analysis of mouse IL-1 beta matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA721976) diluted in carbonate/bicarbonate buffer, at a concentration of 4 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted mouse IL-1 beta protein (HA210706) starting from 3000 pg/ml to 0 pg/ml and detect antibody (HA721977)-Biotin (0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
|
Fig2: The concentrations of IL-1β were interpolated from the IL-1β standard curve and corrected for sample dilution. The mean IL-1β concentration were determined to be 27pg/mL and 1,615 pg/mL in unstimuated and LPS stimulated Raw264.7 cell extract. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.