Human MMP-8 Recombinant Rabbit Monoclonal Antibody [PSH03-34]
cat.: HA721979
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Det) |
| Clonality: | Monoclonal |
| Clone number: | PSH03-34 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 53.4 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human MMP8 aa 21-467 (P22894). |
| Positive control: | Recombinant human MMP8 protein (HA210646). |
| Subcellular location: | Secreted |
| Recommended Dilutions:
ELISA(Det) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH03-33] to Human MMP8 (Capture) (HA721978) and recombinant standard Human MMP8 (HA210646) as the standard. The reference range value is 20.6-5000 pg/ml. |
| Uniprot #: | SwissProt: P22894 Human |
| Alternative names: | CLG 1 CLG1 Collagenase 1 Collagenase 1 neutrophil HNC Matrix metallopeptidase 8 (neutrophil collagenase) Matrix metalloprotease 8 Matrix metalloproteinase-8 MMP 8 MMP-8 Mmp8 MMP8_HUMAN Neutrophil collagenase PMNL CL PMNL collagenase PMNL-CL PMNLCL |
Images
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Fig1:
Sandwich ELISA analysis of human MMP8 matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA721978) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted human MMP8 protein (HA210646) starting from 5000 pg/ml to 0 pg/ml and detect antibody (HA721979, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2: The concentrations of MMP8 were measured in duplicates, interpolated from the MMP8 standard curves and corrected for sample dilution. The mean MMP8 concentration was determined to be 1043.74 pg/mL in A375 supernatant. |
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Fig3: The concentrations of MMP8 were measured in duplicates, interpolated from the MMP8 standard curves and corrected for sample dilution. The mean MMP8 concentration was determined to be 24841.38 pg/mL in SK-Mel-28 supernatant. |
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Fig4: The concentrations of MMP8 were measured in duplicates, interpolated from the MMP8 standard curves and corrected for sample dilution. Undiluted sample is mixed human serum from twenty volunteers 1%. The mean MMP8 concentration was determined to be 240.35 ng/mL in mixed human serum from twenty volunteers. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.