Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
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Species reactivity: | Human, Mouse, Rat |
Applications: | WB, IF-Cell, FC |
Clonality: | Monoclonal |
Clone number: | PSH03-39 |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 1ug/ul |
Purification: | Protein A affinity purified. |
Molecular weight: | Predicted band size: 83 kDa |
Isotype: | IgG |
Immunogen: | Recombinant protein within human MAPK6 aa 1-350 / 721. |
Positive control: | HeLa cell lysate, A431 cell lysate, A549 cell lysate, PC-3M cell lysate, C2C12 cell lysate, NIH/3T3 cell lysate, C6 cell lysate, 293T, C2C12, C6. |
Subcellular location: | Cytoplasm, Nucleus. |
Recommended Dilutions:
WB IF-Cell FC |
1:2,000 1:100 1:1,000 |
Uniprot #: | SwissProt: Q16659 Human | Q61532 Mouse | P27704 Rat |
Alternative names: | ERK-3 ERK3 Extracellular signal regulated kinase 3 Extracellular signal regulated kinase p97 Extracellular signal-regulated kinase 3 HsT17250 MAP kinase 6 MAP kinase isoform p97 MAPK 6 MAPK6 Mitogen activated protein kinase 6 Mitogen-activated protein kinase 6 MK06_HUMAN p97 MAPK p97-MAPK PRKM6 Protein kinase mitogen activated 5 Protein kinase mitogen activated 6 |
Fig1:
Western blot analysis of MAPK6/ERK3 on different lysates with Rabbit anti-MAPK6/ERK3 antibody (HA721984) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: A431 cell lysate Lane 3: A549 cell lysate Lane 4: PC-3M cell lysate Lane 5: C2C12 cell lysate Lane 6: NIH/3T3 cell lysate Lane 7: C6 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 83 kDa Observed band size: 105 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721984) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunocytochemistry analysis of 293T cells labeling MAPK6/ERK3 with Rabbit anti-MAPK6/ERK3 antibody (HA721984) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-MAPK6/ERK3 antibody (HA721984) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
Fig3:
Immunocytochemistry analysis of C2C12 cells labeling MAPK6/ERK3 with Rabbit anti-MAPK6/ERK3 antibody (HA721984) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-MAPK6/ERK3 antibody (HA721984) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
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Fig4:
Immunocytochemistry analysis of C6 cells labeling MAPK6/ERK3 with Rabbit anti-MAPK6/ERK3 antibody (HA721984) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-MAPK6/ERK3 antibody (HA721984) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
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Fig5:
Flow cytometric analysis of 293T cells labeling MAPK6/ERK3. Cells were fixed and permeabilized. Then stained with the primary antibody (HA721984, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Fig6:
Flow cytometric analysis of C6 cells labeling MAPK6/ERK3. Cells were fixed and permeabilized. Then stained with the primary antibody (HA721984, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |