HA721985

Peroxiredoxin 4 Recombinant Rabbit Monoclonal Antibody [PSH03-40]

cat.: HA721985

Product Type:	Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity:	Human
Applications:	WB, IF-Cell, IHC-P
Clonality:	Monoclonal
Clone number:	PSH03-40

Form:	Liquid
Storage condition:	Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage buffer:	PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration:	1ug/ul
Purification:	Protein A affinity purified.
Molecular weight:	Predicted band size: 31 kDa
Isotype:	IgG
Immunogen:	Recombinant protein within human Peroxiredoxin 4 aa 1-271 / 271.
Positive control:	HeLa cell lysate, HEK-293 cell lysate, HepG2 cell lysate, K-562 cell lysate, Jurkat cell lysate, MCF7 cell lysate, LNCaP cell lysate, HeLa, human breast tissue, human liver tissue, human testis tissue.
Subcellular location:	Cytoplasm, Endoplasmic reticulum.
Recommended Dilutions: WB IF-Cell IHC-P	1:2,000 1:100 1:500
Uniprot #:	SwissProt: Q13162 Human
Alternative names:	Antioxidant enzyme 372 Antioxidant enzyme AOE372 AOE37 2 AOE37-2 AOE372 EC 1.11.1.15 Peroxiredoxin IV Peroxiredoxin-4 Peroxiredoxin4 PRDX 4 Prdx4 PRDX4_HUMAN PRX 4 Prx IV Prx-IV PRX4 PrxIV Thioredoxin dependent peroxide reductase A0372 Thioredoxin Peroxidase (Antioxidant Enzyme) Thioredoxin peroxidase Thioredoxin peroxidase AO372 Thioredoxin-dependent peroxide reductase A0372 TRANK

Images

Fig1: Western blot analysis of Peroxiredoxin 4 on different lysates with Rabbit anti-Peroxiredoxin 4 antibody (HA721985) at 1/2,000 dilution.

Lane 1: HeLa cell lysate
Lane 2: HEK-293 cell lysate
Lane 3: HepG2 cell lysate
Lane 4: K-562 cell lysate
Lane 5: Jurkat cell lysate
Lane 6: MCF7 cell lysate
Lane 7: LNCaP cell lysate

Lysates/proteins at 20 µg/Lane.

Predicted band size: 31 kDa
Observed band size: 27 kDa

Exposure time: 50 seconds; ECL: K1801;
4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721985) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Fig2: Western blot analysis of Peroxiredoxin 4 on different lysates with Rabbit anti-Peroxiredoxin 4 antibody (HA721985) at 1/5,000 dilution.

Lane 1: HAP1-parental cell lysate
Lane 2: HAP1-Peroxiredoxin 4 KD cell lysate

Lysates/proteins at 10 µg/Lane.

Predicted band size: 31 kDa
Observed band size: 27 kDa

Exposure time: 30 seconds; ECL: K1801;
4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721985) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

	Fig3: Immunocytochemistry analysis of HeLa cells labeling Peroxiredoxin 4 with Rabbit anti-Peroxiredoxin 4 antibody (HA721985) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Peroxiredoxin 4 antibody (HA721985) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
	Fig4: Immunohistochemical analysis of paraffin-embedded human breast tissue with Rabbit anti-Peroxiredoxin 4 antibody (HA721985) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA721985) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig5: Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-Peroxiredoxin 4 antibody (HA721985) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA721985) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Fig6: Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-Peroxiredoxin 4 antibody (HA721985) at 1/500 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA721985) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.