Dnmt2 Recombinant Rabbit Monoclonal Antibody [PSH03-43]
cat.: HA721988
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Monkey |
| Applications: | WB, IF-Cell, IHC-P, FC |
| Clonality: | Monoclonal |
| Clone number: | PSH03-43 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 45 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human Dnmt2 aa 1-391 / 391. |
| Positive control: | MDA-MB-231 cell lysate, HeLa cell lysate, 293T cell lysate, HCT 116 cell lysate, U-2 OS cell lysate, COS-1 cell lysate, MCF7, human testis tissue. |
| Subcellular location: | Cytoplasm. |
| Recommended Dilutions:
WB IF-Cell IHC-P FC |
1:2,000 1:100 1:2,000 1:1,000 |
| Uniprot #: | SwissProt: O14717 Human |
| Alternative names: | dDNMT DmMT 2 DmMT2 DNA (cytosine 5 ) methyltransferase 2 DNA (cytosine 5) methyltransferase like protein 2 DNA (cytosine-5)-methyltransferase-like protein 2 DNA 5 cytosine methyltransferase DNA methyltransferase 2 DNA methyltransferase homolog HsaIIP DNA MTase homolog HsaIIP Dnmt 2 Dnmt2 M.HsaIIP MHsaIIP nmt 2 nmt2 OTTHUMP00000045198 PuMet RNMT 1 RNMT1 TRDMT 1 TRDMT_HUMAN TRDMT1 tRNA (cytosine 5 ) methyltransferase tRNA (cytosine(38)-C(5))-methyltransferase tRNA aspartic acid methyltransferase 1 tRNA aspartic acid methyltransferase 1 variant 1 tRNA aspartic acid methyltransferase 1 variant 2 tRNA aspartic acid methyltransferase 1 variant 3 tRNA aspartic acid methyltransferase 1 variant 4 tRNA aspartic acid methyltransferase 1 variant 5 tRNA aspartic acid methyltransferase 1 variant 8 |
Images
|
Fig1:
Western blot analysis of Dnmt2 on different lysates with Rabbit anti-Dnmt2 antibody (HA721988) at 1/2,000 dilution. Lane 1: MDA-MB-231 cell lysate Lane 2: HeLa cell lysate Lane 3: 293T cell lysate Lane 4: HCT 116 cell lysate Lane 5: U-2 OS cell lysate Lane 6: COS-1 cell lysate Lysates/proteins at 30 µg/Lane. Predicted band size: 45 kDa Observed band size: 42 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721988) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of Dnmt2 on different lysates with Rabbit anti-Dnmt2 antibody (HA721988) at 1/5,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-Dnmt2 KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 45 kDa Observed band size: 42 kDa Exposure time: 20 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721988) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig3:
Immunocytochemistry analysis of MCF7 cells labeling Dnmt2 with Rabbit anti-Dnmt2 antibody (HA721988) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Dnmt2 antibody (HA721988) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. |
|
Fig4:
Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-Dnmt2 antibody (HA721988) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721988) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig5:
Flow cytometric analysis of MCF7 cells labeling Dnmt2. Cells were fixed and permeabilized. Then stained with the primary antibody (HA721988, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.