Glutathione Reductase Recombinant Rabbit Monoclonal Antibody [JE31-42]
cat.: HA721989
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JE31-42 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 56 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human Glutathione Reductase aa 473-522 / 522. |
| Positive control: | HepG2 cell lysate, A549 cell lysate, K-562 cell lysate, Jurkat cell lysate, mouse liver tissue lysate, mouse brain tissue lysate, mouse kidney tissue lysate, rat lung tissue lysate, rat kidney tissue lysate. |
| Subcellular location: | Mitochondrion; Cytoplasm. |
| Recommended Dilutions:
WB |
1:2,000 |
| Uniprot #: | SwissProt: P00390 Human | P47791 Mouse | P70619 Rat |
| Alternative names: | GLR1 GLUR Glutathione reductase Glutathione reductase mitochondrial Glutathione reductase, mitochondrial GR Gr1 GRase GRD 1 GRD1 GSHR_HUMAN GSR MGC78522 |
Images
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Fig1:
Western blot analysis of Glutathione Reductase on different lysates with Rabbit anti-Glutathione Reductase antibody (HA721989) at 1/2,000 dilution. Lane 1: HepG2 cell lysate (20 µg/Lane) Lane 2: A549 cell lysate (20 µg/Lane) Lane 3: K-562 cell lysate (20 µg/Lane) Lane 4: Jurkat cell lysate (20 µg/Lane) Lane 5: Mouse liver tissue lysate (40 µg/Lane) Lane 6: Mouse brain tissue lysate (40 µg/Lane) Lane 7: Mouse kidney tissue lysate (40 µg/Lane) Lane 8: Rat lung tissue lysate (40 µg/Lane) Lane 9: Rat kidney tissue lysate (40 µg/Lane) Predicted band size: 56 kDa Observed band size: 54 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721989) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of Glutathione Reductase on different lysates with Rabbit anti-Glutathione Reductase antibody (HA721989) at 1/2,000 dilution. Lane 1: HCT 116-si NT cell lysate Lane 2: HCT 116-si Glutathione Reductase cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 56 kDa Observed band size: 54 kDa Exposure time: 20 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721989) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.