IMP3 Recombinant Rabbit Monoclonal Antibody [JE34-04]
cat.: HA721990
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human
Applications: WB, IF-Cell, IHC-P
Clonality: Monoclonal
Clone number: JE34-04
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 64 kDa
Isotype: IgG
Immunogen: Recombinant protein within Human IMP3 aa 1-250 / 579.
Positive control: HeLa cell lysate, Jurkat cell lysate, MDA-MB-231 cell lysate, K-562 cell lysate, HT-29 cell lysate, HeLa, human lung adenocarcinoma tissue, human placenta tissue, human tonsil tissue.
Subcellular location: Nucleus, Cytoplasm, P-body, Stress granule.
Recommended Dilutions:
  WB
  IF-Cell
  IHC-P

1:1,000
1:100
1:200
Uniprot #: SwissProt: O00425 Human
Alternative names: Cancer/testis antigen 98 CT98 DKFZp686F1078 hKOC IF2B3_HUMAN IGF II mRNA binding protein 3 IGF-II mRNA-binding protein 3 IGF2 mRNA binding protein 3 IGF2 mRNA-binding protein 3 IGF2BP3 IMP 3 IMP-3 Insulin like growth factor 2 mRNA binding protein 3 Insulin-like growth factor 2 mRNA-binding protein 3 KH domain containing protein overexpressed in cancer KH domain-containing protein overexpressed in cancer KOC 1 KOC1 VICKZ 3 VICKZ family member 3 VICKZ3
Images
HA721990_1.jpg Fig1: Western blot analysis of IMP3 on different lysates with Rabbit anti-IMP3 antibody (HA721990) at 1/1,000 dilution.

Lane 1: HeLa cell lysate
Lane 2: Jurkat cell lysate
Lane 3: MDA-MB-231 cell lysate
Lane 4: K-562 cell lysate
Lane 5: HT-29 cell lysate

Lysates/proteins at 20 µg/Lane.

Predicted band size: 64 kDa
Observed band size: 70 kDa

Exposure time: 14 seconds; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721990) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
HA721990_2.jpg Fig2: Immunocytochemistry analysis of HeLa cells labeling IMP3 with Rabbit anti-IMP3 antibody (HA721990) at 1/100 dilution.

Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-IMP3 antibody (HA721990) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
HA721990_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human lung adenocarcinoma tissue with Rabbit anti-IMP3 antibody (HA721990) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721990) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA721990_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human placenta tissue with Rabbit anti-IMP3 antibody (HA721990) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721990) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA721990_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-IMP3 antibody (HA721990) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721990) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.