Lyn Recombinant Rabbit Monoclonal Antibody [JE44-75]
cat.: HA721991
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, FC |
| Clonality: | Monoclonal |
| Clone number: | JE44-75 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 59 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human Lyn aa 1-100. |
| Positive control: | Ramos cell lysate, A431 cell lysate, Jurkat cell lysate, THP-1 cell lysate, Raji cell lysate, K-562 cell lysate, U-937 cell lysate, mouse lung tissue lysate, rat lung tissue lysate, A431. |
| Subcellular location: | Cell membrane, Nucleus, Cytoplasm, perinuclear region, Golgi apparatus, Membrane. |
| Recommended Dilutions:
WB FC |
1:1,000-1:2,000 1:1,000 |
| Uniprot #: | SwissProt: P07948 Human | P25911 Mouse | Q07014 Rat |
| Alternative names: | AA407514 EC 2.7.10.2 FLJ26625 Hck 2 JTK 8 JTK8 Lck/Yes related novel protein tyrosine kinase LYN LYN proto oncogene, Src family tyrosine kinase LYN_HUMAN ONCOGENE LYN p53Lyn p56Lyn Tyrosine protein kinase LYN Tyrosine-protein kinase Lyn V yes 1 Yamaguchi sarcoma viral related oncogene homolog Yamaguchi sarcoma viral (v yes 1) related oncogene homolog |
Images
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Fig1:
Western blot analysis of Lyn on different lysates with Rabbit anti-Lyn antibody (HA721991) at 1/1,000 dilution. Lane 1: Ramos cell lysate (15 µg/Lane) Lane 2: A431 cell lysate (15 µg/Lane) Lane 3: Jurkat cell lysate (15 µg/Lane) Lane 4: THP-1 cell lysate (15 µg/Lane) Lane 5: Raji cell lysate (15 µg/Lane) Lane 6: K-562 cell lysate (15 µg/Lane) Lane 7: U-937 cell lysate (15 µg/Lane) Lane 8: Mouse lung tissue lysate (30 µg/Lane) Lane 9: Rat lung tissue lysate (30 µg/Lane) Predicted band size: 59 kDa Observed band size: 55 kDa Exposure time: 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721991) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of Lyn on different lysates with Rabbit anti-Lyn antibody (HA721991) at 1/2,000 dilution. Lane 1: A549-si NT cell lysate Lane 2: A549-si Lyn cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 59 kDa Observed band size: 55 kDa Exposure time: 1 minute; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721991) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig3:
Flow cytometric analysis of A431 cells labeling Lyn. Cells were fixed and permeabilized. Then stained with the primary antibody (HA721991, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.