DOCK2 Recombinant Rabbit Monoclonal Antibody [JE53-62]
cat.: HA721993
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human
Applications: WB
Clonality: Monoclonal
Clone number: JE53-62
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 212 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human DOCK2 aa 1,781-1,830 / 1,830.
Positive control: Jurkat cell lysate, THP-1 cell lysate, Raji cell lysate.
Subcellular location: Endomembrane system, Cytoplasm, cytoskeleton.
Recommended Dilutions:
  WB

1:2,000
Uniprot #: SwissProt: Q92608 Human
Alternative names: Dedicator of cyto kinesis 2 Dedicator of cytokinesis 2 Dedicator of cytokinesis protein 2 DOCK 2 FLJ46592 IMD40 KIAA0209
Images
HA721993_1.jpg Fig1: Western blot analysis of DOCK2 on different lysates with Rabbit anti-DOCK2 antibody (HA721993) at 1/2,000 dilution.

Lane 1: Jurkat cell lysate
Lane 2: THP-1 cell lysate
Lane 3: Raji cell lysate
Lane 4: 293T cell lysate (negative)

Lysates/proteins at 20 µg/Lane.

Predicted band size: 212 kDa
Observed band size: 180 kDa

Exposure time: 3 minutes;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721993) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.