DOCK2 Recombinant Rabbit Monoclonal Antibody [JE53-62]
cat.: HA721993
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JE53-62 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 212 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human DOCK2 aa 1,781-1,830 / 1,830. |
| Positive control: | Jurkat cell lysate, THP-1 cell lysate, Raji cell lysate. |
| Subcellular location: | Endomembrane system, Cytoplasm, cytoskeleton. |
| Recommended Dilutions:
WB |
1:2,000 |
| Uniprot #: | SwissProt: Q92608 Human |
| Alternative names: | Dedicator of cyto kinesis 2 Dedicator of cytokinesis 2 Dedicator of cytokinesis protein 2 DOCK 2 FLJ46592 IMD40 KIAA0209 |
Images
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Fig1:
Western blot analysis of DOCK2 on different lysates with Rabbit anti-DOCK2 antibody (HA721993) at 1/2,000 dilution. Lane 1: Jurkat cell lysate Lane 2: THP-1 cell lysate Lane 3: Raji cell lysate Lane 4: 293T cell lysate (negative) Lysates/proteins at 20 µg/Lane. Predicted band size: 212 kDa Observed band size: 180 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721993) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.