Leukotriene B4 Receptor Recombinant Rabbit Monoclonal Antibody [JE58-77]
cat.: HA721994
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JE58-77 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 38 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human Leukotriene B4 Receptor aa 303-352 / 352. |
| Positive control: | MCF7 cell lysate, MDA-MB-468 cell lysate, mouse skin tissue lysate, mouse lung tissue lysate, rat lung tissue lysate, human breast cancer tissue, human esophagus tissue. |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
WB IHC-P |
1:1,000 1:200-1:1,000 |
| Uniprot #: | SwissProt: Q15722 Human | O88855 Mouse | Q9R0Q2 Rat |
| Alternative names: | BLT BLT1 BLTR Chemoattractant receptor-like 1 Chemokine receptor like 1 CMKRL1 G protein coupled receptor 16 G-protein coupled receptor 16 GPR16 Leukotriene B4 G Protein coupled receptor Leukotriene B4 receptor 1 Leukotriene B4 receptor LT4R1_HUMAN LTB4 R 1 LTB4-R 1 LTB4-R1 LTB4R 1 Ltb4r LTB4R1 LTBR1 P2RY7 P2Y purinoceptor 7 P2Y7 Purinergic receptor P2Y G protein coupled 7 |
Images
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Fig1:
Western blot analysis of Leukotriene B4 Receptor on different lysates with Rabbit anti-Leukotriene B4 Receptor antibody (HA721994) at 1/1,000 dilution. Lane 1: MCF7 cell lysate Lane 2: MDA-MB-468 cell lysate (no heat) Lysates/proteins at 20 µg/Lane. Predicted band size: 38 kDa Observed band size: 38 kDa Exposure time: 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721994) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Western blot analysis of Leukotriene B4 Receptor on different lysates with Rabbit anti-Leukotriene B4 Receptor antibody (HA721994) at 1/1,000 dilution. Lane 1: Mouse skin tissue lysate (hot lysis) Lane 2: Mouse lung tissue lysate (no heat) Lane 3: Rat lung tissue lysate (no heat) Lysates/proteins at 40 µg/Lane. Predicted band size: 38 kDa Observed band size: 38 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721994) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Rabbit anti-Leukotriene B4 Receptor antibody (HA721994) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721994) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded human esophagus tissue with Rabbit anti-Leukotriene B4 Receptor antibody (HA721994) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721994) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.