| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JE59-73 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 15 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human Substance P aa 41-90 / 129. |
| Positive control: | Rat spinal cord tissue. |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
IHC-P |
1:200 |
| Uniprot #: | SwissProt: P20366 Human | P41539 Mouse | P06767 Rat |
| Alternative names: | C-terminal-flanking peptide Hs.2563 neurokinin 1 neurokinin 2 neurokinin A neurokinin alpha Neuromedin L neuropeptide gamma neuropeptide K NK2 NKA NKNA NPK PPT preprotachykinin protachykinin protachykinin-1 Substance K SubstanceP TAC1 TAC2 TAC2, formerly Tachykinin 1 tachykinin 2 tachykinin 2, formerly Tachykinin precursor 1 tachykinin, precursor 1 (substance K, substance P, neurokinin 1, neurokinin 2, neuromedin L, neurokinin alpha, neuropeptide K, neuropeptide gamma) Tachykinin1 TKN1_HUMAN |
|
Fig1:
Immunohistochemical analysis of paraffin-embedded rat spinal cord tissue with Rabbit anti-Substance P antibody (HA721995) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721995) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |