Substance P Recombinant Rabbit Monoclonal Antibody [JE59-73]
cat.: HA721995
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: IHC-P
Clonality: Monoclonal
Clone number: JE59-73
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 15 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human Substance P aa 41-90 / 129.
Positive control: Rat spinal cord tissue.
Subcellular location: Secreted.
Recommended Dilutions:
  IHC-P

1:200
Uniprot #: SwissProt: P20366 Human | P41539 Mouse | P06767 Rat
Alternative names: C-terminal-flanking peptide Hs.2563 neurokinin 1 neurokinin 2 neurokinin A neurokinin alpha Neuromedin L neuropeptide gamma neuropeptide K NK2 NKA NKNA NPK PPT preprotachykinin protachykinin protachykinin-1 Substance K SubstanceP TAC1 TAC2 TAC2, formerly Tachykinin 1 tachykinin 2 tachykinin 2, formerly Tachykinin precursor 1 tachykinin, precursor 1 (substance K, substance P, neurokinin 1, neurokinin 2, neuromedin L, neurokinin alpha, neuropeptide K, neuropeptide gamma) Tachykinin1 TKN1_HUMAN
Images
HA721995_1.jpg Fig1: Immunohistochemical analysis of paraffin-embedded rat spinal cord tissue with Rabbit anti-Substance P antibody (HA721995) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721995) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.