PRMT7 Recombinant Rabbit Monoclonal Antibody [JE64-78]
cat.: HA721996
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, IP |
| Clonality: | Monoclonal |
| Clone number: | JE64-78 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 78 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human PRMT7 aa 1-50 / 692. |
| Positive control: | HeLa cell lysate, Jurkat cell lysate, HepG2 cell lysate, HEK-293 cell lysate, 293T cell lysate, MCF7 cell lysate, NIH/3T3 cell lysate, mouse testis tissue lysate, rat testis tissue lysate, mouse testis tissue, rat testis tissue. |
| Subcellular location: | Cytoplasm, cytosol, Nucleus |
| Recommended Dilutions:
WB IHC-P IP |
1:1,000 1:1,000 1-2μg/sample |
| Uniprot #: | SwissProt: Q9NVM4 Human | Q922X9 Mouse | Q5U4E8 Rat |
| Alternative names: | [Myelin basic protein]-arginine N-methyltransferase PRMT7 ANM7_HUMAN FLJ10640 Histone-arginine N-methyltransferase PRMT7 KIAA1933 Myelin basic protein arginine N methyltransferase OTTHUMP00000174863 PRMT7 Protein arginine methyltransferase 7 Protein arginine N-methyltransferase 7 |
Images
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Fig1:
Western blot analysis of PRMT7 on different lysates with Rabbit anti-PRMT7 antibody (HA721996) at 1/1,000 dilution. Lane 1: HeLa cell lysate (15 µg/Lane) Lane 2: Jurkat cell lysate (15 µg/Lane) Lane 3: HepG2 cell lysate (15 µg/Lane) Lane 4: HEK-293 cell lysate (15 µg/Lane) Lane 5: 293T cell lysate (15 µg/Lane) Lane 6: MCF7 cell lysate (15 µg/Lane) Lane 7: NIH/3T3 cell lysate (15 µg/Lane) Lane 8: Mouse testis tissue lysate (30 µg/Lane) Lane 9: Rat testis tissue lysate (30 µg/Lane) Predicted band size: 78 kDa Observed band size: 78 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721996) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded mouse testis tissue with Rabbit anti-PRMT7 antibody (HA721996) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721996) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded rat testis tissue with Rabbit anti-PRMT7 antibody (HA721996) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721996) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
PRMT7 was immunoprecipitated from 0.2 mg mouse testis tissue lysate with HA721996 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA721996 at 1/1,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: mouse testis tissue lysate (input) Lane 2: HA721996 IP in mouse testis tissue lysate Lane 3: Rabbit IgG instead of HA721996 in mouse testis tissue lysate Blocking/Dilution buffer: primary antibody dilution (K1803) Exposure time: 11 seconds; ECL: K1801 |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.