GCSF Receptor Recombinant Rabbit Monoclonal Antibody [PSH03-44]
cat.: HA721998
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | PSH03-44 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 92 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human GCSF Receptor aa 651-836 / 836. |
| Positive control: | A431 cell lysate, K-562 cell lysate, THP-1 cell lysate, Jurkat cell lysate, Ramos cell lysate, mouse placenta tissue lysate, mouse brain tissue lysate, mouse testis tissue lysate, rat placenta tissue lysate, rat brain tissue lysate, rat testis tissue lysate, rat skin tissue lysate. |
| Subcellular location: | Cell membrane; Secreted. |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: Q99062 Human | P40223 Mouse Entrez Gene: 298518 Rat |
| Alternative names: | CD 114 CD114 CD114 antigen Colony stimulating factor 3 receptor (granulocyte) Colony stimulating factor 3 receptor CSF 3R CSF3R CSF3R_HUMAN Csfgr G CSF R G-CSF receptor G-CSF-R GCSFR Granulocyte colony stimulating factor receptor Granulocyte colony-stimulating factor receptor OTTHUMP00000009703 OTTHUMP00000009704 OTTHUMP00000009705 |
Images
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Fig1:
Western blot analysis of GCSF Receptor on different lysates with Rabbit anti-GCSF Receptor antibody (HA721998) at 1/2,000 dilution. Lane 1: A431 cell lysate (20 µg/Lane) Lane 2: K-562 cell lysate (20 µg/Lane) Lane 3: THP-1 cell lysate (no heat) (20 µg/Lane) Lane 4: Jurkat cell lysate (20 µg/Lane) Lane 5: Ramos cell lysate (20 µg/Lane) Lane 6: Mouse placenta tissue lysate (40 µg/Lane) Lane 7: Mouse brain tissue lysate (no heat) (40 µg/Lane) Lane 8: Mouse testis tissue lysate (40 µg/Lane) Lane 9: Rat placenta tissue lysate (40 µg/Lane) Lane 10: Rat brain tissue lysate (no heat) (40 µg/Lane) Lane 11: Rat testis tissue lysate (40 µg/Lane) Lane 12: Rat skin tissue lysate (no heat) (40 µg/Lane) Predicted band size: 92 kDa Observed band size: 50-110 kDa Exposure time: 1 minute 40 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721998) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.