TRADD Recombinant Rabbit Monoclonal Antibody [JE32-60]
cat.: HA722000
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell |
| Clonality: | Monoclonal |
| Clone number: | JE32-60 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 34 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human TRADD aa 163-312 / 312. |
| Positive control: | HeLa cell lysate, Jurkat cell lysate, RAW264.7 cell lysate, C6 cell lysate, PC-12 cell lysate, Mouse spleen tissue lysate, Rat spleen tissue lysate, HL-60 cell lysate, K-562 cell lysate, Ramos cell lysate, C2C12 cell lysate, MCF7. |
| Subcellular location: | Nucleus, Cytoplasm, cytoskeleton. |
| Recommended Dilutions:
WB IF-Cell |
1:1,000 1:100 |
| Uniprot #: | SwissProt: Q15628 Human | Q3U0V2 Mouse Entrez Gene: 246756 Rat |
| Alternative names: | 9130005N23Rik AA930854 TNFR1 associated DEATH domain protein TNFR1-associated DEATH domain protein TNFRSF1A associated via death domain TNFRSF1A-associated via death domain tradd TRADD_HUMAN Tumor necrosis factor receptor type 1 associated DEATH domain protein Tumor necrosis factor receptor type 1-associated DEATH domain protein |
Images
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Fig1:
Western blot analysis of TRADD on different lysates with Rabbit anti-TRADD antibody (HA722000) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 µg/Lane) Lane 2: Jurkat cell lysate (20 µg/Lane) Lane 3: RAW264.7 cell lysate (20 µg/Lane) Lane 4: C6 cell lysate (20 µg/Lane) Lane 5: PC-12 cell lysate (20 µg/Lane) Lane 6: Mouse spleen tissue lysate (40 µg/Lane) Lane 7: Rat spleen tissue lysate (40 µg/Lane) Predicted band size: 34 kDa Observed band size: 34 kDa Exposure time: 1 minute 50 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722000) at 1/1,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Western blot analysis of TRADD on different lysates with Rabbit anti-TRADD antibody (HA722000) at 1/1,000 dilution. Lane 1: HL-60 cell lysate (20 µg/Lane) Lane 2: HeLa cell lysate (20 µg/Lane) Lane 3: K-562 cell lysate (20 µg/Lane) Lane 4: Ramos cell lysate (20 µg/Lane) Lane 5: Jurkat cell lysate (20 µg/Lane) Lane 6: C2C12 cell lysate (20 µg/Lane) Lane 7: PC-12 cell lysate (20 µg/Lane) Predicted band size: 34 kDa Observed band size: 34 kDa Exposure time: 2 minutes 18 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722000) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig3:
Immunocytochemistry analysis of MCF7 cells labeling TRADD with Rabbit anti-TRADD antibody (HA722000) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-TRADD antibody (HA722000) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.