TIRAP Recombinant Rabbit Monoclonal Antibody [JE46-35]
cat.: HA722001
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JE46-35 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 24 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human TIRAP aa 1-50 / 221. |
| Positive control: | HeLa cell lysate, U-2 OS cell lysate, RAW264.7 cell lysate, mouse liver tissue lysate. |
| Subcellular location: | Cytoplasm, Cell membrane, Membrane. |
| Recommended Dilutions:
WB |
1:2,000 |
| Uniprot #: | SwissProt: P58753 Human | Q99JY1 Mouse |
| Alternative names: | Adapter protein wyatt FLJ42305 MAL MyD88 adapter like protein TIR domain containing adapter protein Toll interleukin 1 receptor (TIR) domain containing adaptor protein Toll interleukin 1 receptor domain containing adapter protein Toll like receptor adaptor protein Wyatt |
Images
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Fig1:
Western blot analysis of TIRAP on different lysates with Rabbit anti-TIRAP antibody (HA722001) at 1/2,000 dilution. Lane 1: HeLa cell lysate (20 µg/Lane) Lane 2: U-2 OS cell lysate (20 µg/Lane) Lane 3: RAW264.7 cell lysate (20 µg/Lane) Lane 4: Mouse liver tissue lysate (40 µg/Lane) Predicted band size: 24 kDa Observed band size: 30 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722001) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.