CBLB Recombinant Rabbit Monoclonal Antibody [PSH03-49]
cat.: HA722008
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | PSH03-49 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 109 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human CBLB aa 400-982 / 982. |
| Positive control: | Jurkat cell lysate, K-562 cell lysate, U-87 MG cell lysate, HeLa cell lysate, A431 cell lysate, A549 cell lysate, NIH/3T3 cell lysate, RAW264.7 cell lysate, PC-12 cell lysate, mouse lung tissue lysate, human stomach tissue. |
| Subcellular location: | Cytoplasm. |
| Recommended Dilutions:
WB IHC-P |
1:2,000 1:200 |
| Uniprot #: | SwissProt: Q13191 Human | Q3TTA7 Mouse | Q8K4S7 Rat |
| Alternative names: | AI429560 AI851073 Cas Br M (murine) ecotropic retroviral transforming sequence b Casitas B lineage lymphoma b Casitas B lineage lymphoma proto oncogene b Casitas B-lineage lymphoma proto-oncogene b Cbl b CBLB CBLB_HUMAN DKFZp686J10223 DKFZp779A0729 DKFZp779F1443 E3 ubiquitin protein ligase CBL B E3 ubiquitin-protein ligase CBL-B EC 6.3.2.- FLJ36865 FLJ41152 Nbla00127 RING finger protein 56 RNF56 SH3 binding protein CBL B SH3-binding protein CBL-B Signal transduction protein CBL B Signal transduction protein CBL-B |
Images
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Fig1:
Western blot analysis of CBLB on different lysates with Rabbit anti-CBLB antibody (HA722008) at 1/2,000 dilution. Lane 1: Jurkat cell lysate (20 µg/Lane) Lane 2: K-562 cell lysate (20 µg/Lane) Lane 3: U-87 MG cell lysate (20 µg/Lane) Lane 4: HeLa cell lysate (20 µg/Lane) Lane 5: A431 cell lysate (20 µg/Lane) Lane 6: A549 cell lysate (20 µg/Lane) Lane 7: NIH/3T3 cell lysate (20 µg/Lane) Lane 8: RAW264.7 cell lysate (20 µg/Lane) Lane 9: PC-12 cell lysate (20 µg/Lane) Lane 10: Mouse lung tissue lysate (40 µg/Lane) Predicted band size: 109 kDa Observed band size: 109 kDa Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722008) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human stomach tissue with Rabbit anti-CBLB antibody (HA722008) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722008) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.