ADAMTS5 Recombinant Rabbit Monoclonal Antibody [PSH03-52]
cat.: HA722011
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, FC |
| Clonality: | Monoclonal |
| Clone number: | PSH03-52 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 102 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human ADAMTS5 aa 251-500 / 930. |
| Positive control: | HepG2 cell lysate, LO2 cell lysate, Huh7 cell lysate, HeLa cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, human liver tissue lysate, mouse placenta tissue lysate, mouse uterus tissue lysate, HepG2, NIH/3T3. |
| Subcellular location: | Secreted, extracellular space, extracellular matrix. |
| Recommended Dilutions:
WB IF-Cell FC |
1:2,000 1:100-1:250 1:1,000 |
| Uniprot #: | SwissProt: Q9UNA0 Human | Q9R001 Mouse Entrez Gene: 304135 Rat |
| Alternative names: | A disintegrin and metalloproteinase with thrombospondin motifs 11 A disintegrin and metalloproteinase with thrombospondin motifs 5 A disintegrin like and metalloprotease (reprolysin type) with thrombospondin type 1 motif 5 A disintegrin like and metalloprotease (reprolysin type) with thrombospondin type 1 motif, 5 (aggrecanase 2) A disintegrin-like and metalloprotease with thrombospondin type 1 motif, 5 A Disintigrin And Metalloproteinase with ThromboSpondin motif-5 ADAM metallopeptidase with thrombospondin type 1 motif 5 ADAM TS 11 ADAM TS 5 ADAM TS5 ADAM-TS 11 ADAM-TS 5 ADAM-TS5 ADAMTS 11 ADAMTS 5 ADAMTS-11 ADAMTS-5 ADAMTS11 ADAMTS11, formerly Adamts5 ADMP 2 ADMP-2 ADMP2 Aggrecanase 2 Aggrecanase-2 ATS5_HUMAN FLJ36738 Implantin ThromboSpondin motif-5 |
Images
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Fig1:
Western blot analysis of ADAMTS5 on different lysates with Rabbit anti-ADAMTS5 antibody (HA722011) at 1/2,000 dilution. Lane 1: HepG2 cell lysate Lane 2: LO2 cell lysate Lane 3: Huh7 cell lysate Lane 4: HeLa cell lysate Lane 5: NIH/3T3 cell lysate Lane 6: PC-12 cell lysate Lane 7: Human liver tissue lysate Lane 8: Mouse placenta tissue lysate Lane 9: Mouse uterus tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 102 kDa Observed band size: 75 kDa Exposure time: 50 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722011) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunocytochemistry analysis of HepG2 cells labeling ADAMTS5 with Rabbit anti-ADAMTS5 antibody (HA722011) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-ADAMTS5 antibody (HA722011) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
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Fig3:
Immunocytochemistry analysis of NIH/3T3 cells labeling ADAMTS5 with Rabbit anti-ADAMTS5 antibody (HA722011) at 1/250 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-ADAMTS5 antibody (HA722011) at 1/250 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
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Fig4:
Flow cytometric analysis of HepG2 cells labeling ADAMTS5. Cells were fixed and permeabilized. Then stained with the primary antibody (HA722011, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
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Fig5:
Flow cytometric analysis of NIH/3T3 cells labeling ADAMTS5. Cells were fixed and permeabilized. Then stained with the primary antibody (HA722011, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
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