Patched / PTCH1 Recombinant Rabbit Monoclonal Antibody [PSH03-53]
cat.: HA722012
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | PSH03-53 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 161 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human PTCH1 aa 122-436 / 1,447. |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
WB |
1:10,000 |
| Uniprot #: | SwissProt: Q13635 Human |
| Alternative names: | A230106A15Rik BCNS FLJ26746 FLJ42602 Holoprosencephaly 7 HPE7 mes NBCCS OTTHUMP00000021709 OTTHUMP00000021710 Patched Patched (Drosophila) homolog Patched 1 Patched homolog (Drosophila) Patched homolog 1 (Drosophila) Patched homolog 1 Patched protein homolog 1 Protein patched homolog 1 PTC PTC1 PTC1_HUMAN PTCH PTCH protein +12b PTCH protein +4' PTCH protein -10 PTCH protein ptch1 PTCH1 protein PTCH11 Ptch2 |
Images
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Fig1:
Western blot analysis of Patched / PTCH1 on different lysates with Rabbit anti-Patched / PTCH1 antibody (HA722012) at 1/10,000 dilution. Lane 1: 293T-NT cell lysate Lane 2: 293T-OE-PTCH1 cell lysate Lysates/proteins at 1 µg/Lane. Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722012) at 1/10,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.