CENPE Recombinant Rabbit Monoclonal Antibody [PSH03-54]
cat.: HA722013
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | PSH03-54 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 316 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human CENPE aa 1-400 / 2,701. |
| Positive control: | HeLa cell lysate, K-562 cell lysate, HEK-293 cell lysate. |
| Subcellular location: | Chromosome, centromere, kinetochore, Cytoplasm, cytoskeleton, spindle. |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: Q02224 Human |
| Alternative names: | CENP E CENP-E CENPE CENPE_HUMAN Centromere associated protein E Centromere autoantigen E (312kD) Centromere autoantigen E Centromere protein E 312kDa Centromere protein E Centromere-associated protein E KIF10 Kinesin family member 10 Kinesin related protein Kinesin related protein CENPE Kinesin-related protein CENPE PPP1R61 Protein phosphatase 1, regulatory subunit 61 |
Images
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Fig1:
Western blot analysis of CENPE on different lysates with Rabbit anti-CENPE antibody (HA722013) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: K-562 cell lysate Lane 3: HEK-293 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 316 kDa Observed band size: 316 kDa Exposure time: 43 seconds; 3-8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722013) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.