MELK Recombinant Rabbit Monoclonal Antibody [PSH03-55]
cat.: HA722014
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | PSH03-55 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 75 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human MELK aa 1-400 / 651. |
| Positive control: | HeLa cell lysate, 293T cell lysate, MDA-MB-231 cell lysate, HCT 116 cell lysate, NIH/3T3 cell lysate, C2C12 cell lysate, human breast cancer tissue, human testis tissue. |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
WB IHC-P |
1:2,000 1:500-1:2,000 |
| Uniprot #: | SwissProt: Q14680 Human | Q61846 Mouse |
| Alternative names: | AI327312 hMELK HPK 38 hPK38 KIAA0175 Likely ortholog of maternal embryonic leucine zipper kinase Maternal embryonic leucine zipper kinase MELK MELK_HUMAN mKIAA0175 MPK38 OTTHUMP00000021377 OTTHUMP00000046113 pEg3 kinase Protein kinase Eg3 Protein kinase PK38 RP23 382O11.1 Tyrosine protein kinase MELK |
Images
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Fig1:
Western blot analysis of MELK on different lysates with Rabbit anti-MELK antibody (HA722014) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: 293T cell lysate Lane 3: MDA-MB-231 cell lysate Lane 4: HCT 116 cell lysate Lane 5: NIH/3T3 cell lysate Lane 6: C2C12 cell lysate Lysates/proteins at 30 µg/Lane. Predicted band size: 75 kDa Observed band size: 72 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722014) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/100,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of MELK on different lysates with Rabbit anti-MELK antibody (HA722014) at 1/2,000 dilution. Lane 1: Hepa1-6-si NT cell lysate Lane 2: Hepa1-6-si MELK cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 75 kDa Observed band size: 75 kDa Exposure time: 20 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722014) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Rabbit anti-MELK antibody (HA722014) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722014) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig4:
Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-MELK antibody (HA722014) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722014) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.