| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | PSH03-56 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 117 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human MDA5 aa 250-1,025 / 1,025. |
| Positive control: | THP-1 cell lysate, HepG2 cell lysate, Jurkat cell lysate, Daudi cell lysate, Raji cell lysate, MCF7 cell lysate, HeLa cell lysate, SH-SY5Y cell lysate, A549 cell lysate, A431 cell lysate, mouse pancreas tissue lysate, rat kidney tissue lysate. |
| Subcellular location: | Cytoplasm, Nucleus, Mitochondrion. |
| Recommended Dilutions:
WB |
1:2,000 |
| Uniprot #: | SwissProt: Q9BYX4 Human | Q8R5F7 Mouse Entrez Gene: 499801 Rat |
| Alternative names: | CADM-140 autoantigen Clinically amyopathic dermatomyositis autoantigen 140 kDa DEAD/H (Asp Glu Ala Asp/His) box polypeptide DEAD/H box polypeptide Helicard Helicase with 2 CARD domains Hlcd IDDM 19 IDDM19 IFIH 1 Ifih1 IFIH1_HUMAN Interferon induced helicase C domain containing protein 1 interferon induced with helicase C domain 1 Interferon induced with helicase C domain protein 1 Interferon-induced helicase C domain-containing protein 1 Interferon-induced with helicase C domain protein 1 MDA 5 MDA-5 Melanoma differentiation associated protein 5 Melanoma differentiation-associated gene 5 Melanoma differentiation-associated protein 5 MGC133047 Murabutide down regulated protein Murabutide down-regulated protein RH 116 RH116 RIG I like receptor 2 RLR 2 RNA helicase DEAD box protein 116 RNA helicase-DEAD box protein 116 |
|
Fig1:
Western blot analysis of MDA5 on different lysates with Rabbit anti-MDA5 antibody (HA722015) at 1/2,000 dilution. Lane 1: THP-1 cell lysate (20 µg/Lane) Lane 2: HepG2 cell lysate (20 µg/Lane) Lane 3: Jurkat cell lysate (20 µg/Lane) Lane 4: Daudi cell lysate (20 µg/Lane) Lane 5: Raji cell lysate (20 µg/Lane) Lane 6: MCF7 cell lysate (20 µg/Lane) Lane 7: HeLa cell lysate (20 µg/Lane) Lane 8: SH-SY5Y cell lysate (20 µg/Lane) Lane 9: A549 cell lysate (20 µg/Lane) Lane 10: A431 cell lysate (20 µg/Lane) Lane 11: Mouse pancreas tissue lysate (40 µg/Lane) Lane 12: Rat kidney tissue lysate (40 µg/Lane) Lysates/proteins at 10 µg/Lane. Predicted band size: 117 kDa Observed band size: 140/100 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722015) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |