MDA5 Recombinant Rabbit Monoclonal Antibody [PSH03-56]
cat.: HA722015
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB
Clonality: Monoclonal
Clone number: PSH03-56
Form: Liquid
Storage condition: Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage buffer: PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 117 kDa
Isotype: IgG
Immunogen: Recombinant protein within human MDA5 aa 250-1,025 / 1,025.
Positive control: THP-1 cell lysate, HepG2 cell lysate, Jurkat cell lysate, Daudi cell lysate, Raji cell lysate, MCF7 cell lysate, HeLa cell lysate, SH-SY5Y cell lysate, A549 cell lysate, A431 cell lysate, mouse pancreas tissue lysate, rat kidney tissue lysate.
Subcellular location: Cytoplasm, Nucleus, Mitochondrion.
Recommended Dilutions:
  WB

1:2,000
Uniprot #: SwissProt: Q9BYX4 Human | Q8R5F7 Mouse
Entrez Gene: 499801 Rat
Alternative names: CADM-140 autoantigen Clinically amyopathic dermatomyositis autoantigen 140 kDa DEAD/H (Asp Glu Ala Asp/His) box polypeptide DEAD/H box polypeptide Helicard Helicase with 2 CARD domains Hlcd IDDM 19 IDDM19 IFIH 1 Ifih1 IFIH1_HUMAN Interferon induced helicase C domain containing protein 1 interferon induced with helicase C domain 1 Interferon induced with helicase C domain protein 1 Interferon-induced helicase C domain-containing protein 1 Interferon-induced with helicase C domain protein 1 MDA 5 MDA-5 Melanoma differentiation associated protein 5 Melanoma differentiation-associated gene 5 Melanoma differentiation-associated protein 5 MGC133047 Murabutide down regulated protein Murabutide down-regulated protein RH 116 RH116 RIG I like receptor 2 RLR 2 RNA helicase DEAD box protein 116 RNA helicase-DEAD box protein 116
Images
HA722015_1.jpg Fig1: Western blot analysis of MDA5 on different lysates with Rabbit anti-MDA5 antibody (HA722015) at 1/2,000 dilution.

Lane 1: THP-1 cell lysate (20 µg/Lane)
Lane 2: HepG2 cell lysate (20 µg/Lane)
Lane 3: Jurkat cell lysate (20 µg/Lane)
Lane 4: Daudi cell lysate (20 µg/Lane)
Lane 5: Raji cell lysate (20 µg/Lane)
Lane 6: MCF7 cell lysate (20 µg/Lane)
Lane 7: HeLa cell lysate (20 µg/Lane)
Lane 8: SH-SY5Y cell lysate (20 µg/Lane)
Lane 9: A549 cell lysate (20 µg/Lane)
Lane 10: A431 cell lysate (20 µg/Lane)
Lane 11: Mouse pancreas tissue lysate (40 µg/Lane)
Lane 12: Rat kidney tissue lysate (40 µg/Lane)

Lysates/proteins at 10 µg/Lane.

Predicted band size: 117 kDa
Observed band size: 140/100 kDa

Exposure time: 3 minutes;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722015) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.