p38 gamma/MAPK12 Recombinant Rabbit Monoclonal Antibody [PSH03-58]
cat.: HA722017
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, IF-Cell, FC |
| Clonality: | Monoclonal |
| Clone number: | PSH03-58 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 42 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human MAPK12 aa 1-367 / 367. |
| Positive control: | 293T cell lysate, HeLa cell lysate, K-562 cell lysate, Neuro-2a cell lysate, PC-12 cell lysate, mouse heart tissue lysate, mouse skeletal muscle tissue lysate, rat skeletal muscle tissue lysate, rat heart tissue lysate, human colon cancer tissue, human heart tissue, human skeletal muscle tissue, mouse heart tissue, rat heart tissue, HeLa, Neuro-2a, PC-12. |
| Subcellular location: | Cytoplasm, Nucleus, Mitochondrion. |
| Recommended Dilutions:
WB IHC-P IF-Cell FC |
1:2,000 1:1,000 1:100 1:1,000 |
| Uniprot #: | SwissProt: P53778 Human | O08911 Mouse | Q63538 Rat |
| Alternative names: | ERK 6 ERK-6 ERK6 Extracellular signal-regulated kinase 6 MAP kinase 12 MAP kinase p38 gamma MAPK 12 Mapk12 Mitogen Activated Protein Kinase 12 Mitogen activated protein kinase p38 gamma Mitogen-activated protein kinase 12 Mitogen-activated protein kinase p38 gamma MK12_HUMAN P38 GAMMA P38GAMMA PRKM12 SAPK 3 SAPK-3 SAPK3 Stress Activated Protein Kinase 3 Stress-activated protein kinase 3 |
Images
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Fig1:
Western blot analysis of p38 gamma/MAPK12 on different lysates with Rabbit anti-p38 gamma/MAPK12 antibody (HA722017) at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution. Lane 1: 293T cell lysate Lane 2: HeLa cell lysate Lane 3: K-562 cell lysate Lane 4: Neuro-2a cell lysate Lane 5: PC-12 cell lysate Lane 6: Mouse heart tissue lysate Lane 7: Mouse skeletal muscle tissue lysate Lane 8: Rat skeletal muscle tissue lysate Lane 9: Rat heart tissue lysate Lysates/proteins at 30 µg/Lane. Predicted band size: 42 kDa Observed band size: 42 kDa Exposure time: 40 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722017) at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Rabbit anti-p38 gamma/MAPK12 antibody (HA722017) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722017) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human heart tissue with Rabbit anti-p38 gamma/MAPK12 antibody (HA722017) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722017) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue with Rabbit anti-p38 gamma/MAPK12 antibody (HA722017) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722017) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Immunohistochemical analysis of paraffin-embedded mouse heart tissue with Rabbit anti-p38 gamma/MAPK12 antibody (HA722017) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722017) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig6:
Immunohistochemical analysis of paraffin-embedded rat heart tissue with Rabbit anti-p38 gamma/MAPK12 antibody (HA722017) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722017) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig7:
Immunocytochemistry analysis of HeLa cells labeling p38 gamma/MAPK12 with Rabbit anti-p38 gamma/MAPK12 antibody (HA722017) at 1/100 dilution. Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-p38 gamma/MAPK12 antibody (HA722017) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
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Fig8:
Immunocytochemistry analysis of Neuro-2a cells labeling p38 gamma/MAPK12 with Rabbit anti-p38 gamma/MAPK12 antibody (HA722017) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-p38 gamma/MAPK12 antibody (HA722017) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
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Fig9:
Immunocytochemistry analysis of PC-12 cells labeling p38 gamma/MAPK12 with Rabbit anti-p38 gamma/MAPK12 antibody (HA722017) at 1/100 dilution. Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-p38 gamma/MAPK12 antibody (HA722017) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
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Fig10:
Flow cytometric analysis of HeLa cells labeling p38 gamma/MAPK12. Cells were fixed and permeabilized. Then stained with the primary antibody (HA722017, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
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Fig11:
Flow cytometric analysis of Neuro-2a cells labeling p38 gamma/MAPK12. Cells were fixed and permeabilized. Then stained with the primary antibody (HA722017, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
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Fig12:
Flow cytometric analysis of PC-12 cells labeling p38 gamma/MAPK12. Cells were fixed and permeabilized. Then stained with the primary antibody (HA722017, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
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Fig13:
Western blot analysis of p38 gamma/MAPK12 with Rabbit anti-p38 gamma/MAPK12 antibody (HA722017) at 1/2,000 dilution. Lane 1: Human p38 gamma recombinant protein, 30ng/Lane Lane 2: Human p38 alpha recombinant protein, 50ng/Lane Lane 3: Human p38 delta recombinant protein, 50ng/Lane Lane 4: Human p38 beta recombinant protein, 50ng/Lane Exposure time: 5 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722017) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.