p38 alpha/MAPK14 + p38 beta/MAPK11 + p38 gamma/MAPK12 Recombinant Rabbit Monoclonal Antibody [PSH03-60]
cat.: HA722019
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat, Monkey |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | PSH03-60 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 42 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human MAPK12 aa 1-367 / 367. |
| Positive control: | 293T cell lysate, HeLa cell lysate, K-562 cell lysate, MDA-MB-231 cell lysate, COS-1 cell lysate, Neuro-2a cell lysate, PC-12 cell lysate, mouse skeletal muscle tissue lysate, rat skeletal muscle tissue lysate, rat heart tissue lysate. |
| Subcellular location: | Cytoplasm, Nucleus, Mitochondrion. |
| Recommended Dilutions:
WB |
1:2,000 |
| Uniprot #: | SwissProt: Q16539 Human | Q15759 Human | P53778 Human | P47811 Mouse | Q9WUI1 Mouse | O08911 Mouse | P70618 Rat | D4A3U7 Rat | Q63538 Rat |
| Alternative names: | ERK 6 ERK-6 ERK6 Extracellular signal-regulated kinase 6 MAP kinase 12 MAP kinase p38 gamma MAPK 12 Mapk12 Mitogen Activated Protein Kinase 12 Mitogen activated protein kinase p38 gamma Mitogen-activated protein kinase 12 Mitogen-activated protein kinase p38 gamma MK12_HUMAN P38 GAMMA P38GAMMA PRKM12 SAPK 3 SAPK-3 SAPK3 Stress Activated Protein Kinase 3 Stress-activated protein kinase 3 |
Images
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Fig1:
Western blot analysis of p38 alpha/MAPK14 + p38 beta/MAPK11 + p38 gamma/MAPK12 on different lysates with Rabbit anti-p38 alpha/MAPK14 + p38 beta/MAPK11 + p38 gamma/MAPK12 antibody (HA722019) at 1/2,000 dilution. Lane 1: 293T cell lysate (30 µg/Lane) Lane 2: HeLa cell lysate (30 µg/Lane) Lane 3: K-562 cell lysate (30 µg/Lane) Lane 4: MDA-MB-231 cell lysate (30 µg/Lane) Lane 5: COS-1 cell lysate (30 µg/Lane) Lane 6: Neuro-2a cell lysate (30 µg/Lane) Lane 7: PC-12 cell lysate (30 µg/Lane) Lane 8: Mouse skeletal muscle tissue lysate (30 µg/Lane) Lane 9: Rat skeletal muscle tissue lysate (30 µg/Lane) Lane 10: Rat heart tissue lysate (30 µg/Lane) Predicted band size: 42 kDa Observed band size: 42 kDa Exposure time: 40 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722019) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of p38 alpha/MAPK14 + p38 beta/MAPK11 + p38 gamma/MAPK12 with Rabbit anti-p38 alpha/MAPK14 + p38 beta/MAPK11 + p38 gamma/MAPK12 antibody (HA722019) at 1/2,000 dilution. Lane 1: Human p38 gamma recombinant protein, 30ng/Lane Lane 2: Human p38 alpha recombinant protein, 50ng/Lane Lane 3: Human p38 delta recombinant protein, 50ng/Lane Lane 4: Human p38 beta recombinant protein, 50ng/Lane Exposure time: 43 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722019) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.