TNF alpha Recombinant Rabbit Monoclonal Antibody [PS01-07]
cat.: HA722022
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | PS01-07 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 26 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within full length protein. |
| Positive control: | THP-1 treated with 500ng/mL LPS for 24 hours then add 300ng/mL BFA for 20 hours cell lysate, RAW264.7 treated with 100ng/mL LPS for 7 hours add 300ng/mL BFA for 3 hours cell lysate. |
| Subcellular location: | Cell membrane, Secreted. |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: P01375 Human | P06804 Mouse |
| Alternative names: | APC1 APC1 protein Cachectin DIF Differentiation inducing factor Macrophage cytotoxic factor Tnf TNF superfamily member 2 TNF superfamily, member 2 TNF, macrophage derived TNF, monocyte derived TNF-a TNF-alpha TNFA TNFA_HUMAN TNFSF2 Tumor necrosis factor (TNF superfamily member 2) Tumor necrosis factor alpha Tumor necrosis factor Tumor necrosis factor ligand superfamily member 2 Tumor Necrosis Factor, Membrane Form Tumor necrosis factor, soluble form |
Images
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Fig1:
Western blot analysis of TNF alpha on different lysates with Rabbit anti-TNF alpha antibody (HA722022) at 1/1,000 dilution. Lane 1: THP-1 cell lysate Lane 2: THP-1 treated with 500ng/mL LPS for 24 hours then add 300ng/mL BFA for 20 hours cell lysate Lane 3: RAW264.7 cell lysate Lane 4: RAW264.7 treated with 100ng/mL LPS for 7 hours, add 300ng/mL BFA for 3 hours cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 26 kDa Observed band size: 26/50/17 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722022) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.